Longitudinal analysis reveals characteristically high proportions of bacterial vaginosis-associated bacteria and temporal variability of vaginal microbiota in northern pig-tailed macaques (Macaca leonina).
Longitudinal analysis reveals characteristically high proportions of bacterial vaginosis-associated bacteria and temporal variability of vaginal microbiota in northern pig-tailed macaques (Macaca leonina).
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DOI：
10.13918/j.issn.2095-8137.2015.5.285
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发表时间：
2015-09
期刊：
影响因子：
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通讯作者：
Lin Zhu;Ai-Hua Lei;Hong-yi Zheng;Longbao Lyu;Zhigang Zhang;Yong-tang Zheng
中科院分区：
文献类型：
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作者：
Lin Zhu;Ai-Hua Lei;Hong-yi Zheng;Longbao Lyu;Zhigang Zhang;Yong-tang Zheng
复杂而动态的阴道微生物生态系统对宿主的健康和疾病都至关重要。关注阴道微生物群如何影响HIV-1感染的研究在选择适当的动物模型方面可能面临限制。考虑到北方猪尾猕猴（Macaca leonina）对HIV-1感染易感，它们可能是用于阐明阴道微生物群有助于对HIV-1感染的抗性和易感性的机制的最佳动物模型。然而，关于北方猪尾猕猴阴道微生物群的组成和时间变异性知之甚少。在这里，我们提出了一个全面的目录的组成和时间动态的阴道微生物群的两个健康的北方猪尾猕猴超过19周，使用454焦磷酸测序的16 S rRNA基因。我们发现与细菌性阴道病相关的各种厌氧菌的比例非常高。Atopobium和Sneathia是优势属，有趣的是，我们证明了乳杆菌属占主导地位的阴道微生物群的存在。此外，纵向分析表明，阴道微生物群的时间动态是相当个性化的。最后，网络分析显示，阴道pH值可能会影响阴道微生物群的时间动态，这表明阴道细菌群落的受试者间变异性可以反映在物种彼此之间以及随着时间的推移与阴道pH值的相关性曲线的受试者间变异中。我们的研究结果表明，北方猪尾猕猴可能是一个理想的动物模型，在高度多微生物和乳酸杆菌占主导地位的国家的背景下，阴道微生物群影响HIV-1感染的易感性和抵抗力的机制进行前瞻性研究。
The complex and dynamic vaginal microbial ecosystem is critical to both health and disease of the host. Studies focusing on how vaginal microbiota influences HIV-1 infection may face limitations in selecting proper animal models. Given that northern pig-tailed macaques (Macaca leonina) are susceptible to HIV-1 infection, they may be an optimal animal model for elucidating the mechanisms by which vaginal microbiota contributes to resistance and susceptibility to HIV-1 infection. However, little is known about the composition and temporal variability of vaginal microbiota of the northern pig-tailed macaque. Here, we present a comprehensive catalog of the composition and temporal dynamics of vaginal microbiota of two healthy northern pig-tailed macaques over 19 weeks using 454-pyrosequencing of 16S rRNA genes. We found remarkably high proportions of a diverse array of anaerobic bacteria associated with bacterial vaginosis. Atopobium and Sneathia were dominant genera, and interestingly, we demonstrated the presence of Lactobacillus-dominated vaginal microbiota. Moreover, longitudinal analysis demonstrated that the temporal dynamics of the vaginal microbiota were considerably individualized. Finally, network analysis revealed that vaginal pH may influence the temporal dynamics of the vaginal microbiota, suggesting that inter-subject variability of vaginal bacterial communities could be mirrored in inter-subject variation in correlation profiles of species with each other and with vaginal pH over time. Our results suggest that the northern pig-tailed macaque could be an ideal animal model for prospective investigation of the mechanisms by which vaginal microbiota influence susceptibility and resistance to HIV-1 infection in the context of highly polymicrobial and Lactobacillus-dominated states.