Occurrence of antibiotics in the main rivers of Shenzhen, China: Association with antibiotic resistance genes and microbial community
Occurrence of antibiotics in the main rivers of Shenzhen, China: Association with antibiotic resistance genes and microbial community
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中国深圳主要河流中抗生素的出现：与抗生素抗性基因和微生物群落的关联
DOI：
10.1016/j.scitotenv.2018.10.398
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发表时间：
2019-02-25
影响因子：
9.8
通讯作者：
Zheng, Chunmiao
中科院分区：
文献类型：
--
作者：
Qiu, Wenhui;Sun, Jing;Zheng, Chunmiao
对深圳市主要河流地表水和沉积物中抗生素的分布情况进行了调查。31个水样中20种抗生素的总浓度范围为36.510 ~ 1075.687 ng L-1（平均244.992 ng L-1），31个沉积物样品中20种抗生素的总浓度范围为28.124 ~ 2728.810 ng g（-1）（平均680.169 ng g（-1））。STZ和SDZ是水体和沉积物中的主要抗生素，因为它们的浓度高于其他化合物。此外，通过对抗生素耐药基因（ARGs）和微生物群落的综合分析，研究了茅洲河底泥中抗生素的产生对ARGs在微生物群落中的传播和演化的影响。结果表明，在所有样品中，sul 1基因是最丰富的ARG，变形杆菌是最丰富的微生物（37.4-51.7%），其次是拟杆菌（15.3-18.4%）。统计分析揭示了抗生素、ARGs与微生物群落的关系。从bla_d基因与梭菌、磺胺甲恶唑的正相关性可以得出一个明确的结论。这表明抗生素可能与某些细菌中ARGs的表达呈正相关，因此在细菌群落中会发生高繁殖。总体而言，ARG的广泛分布强调了需要进一步研究抗生素作为环境中新出现的污染物的影响机制以及对微生物群落的相关风险。(c)2018爱思唯尔B. V.保留所有权利。
The occurrence and distribution of antibiotics were investigated in surface water and sediment collected from the main rivers of Shenzhen, China. Total concentrations of 20 selected antibiotics ranged from 36.510 to 1075.687 ng L-1 (mean 244.992 ng L-1) in 31 water samples and from 28.124 to 2728.810 ng g(-1) (mean 680.169 ng g(-1)) in 31 sediment samples. Notably, STZ and SDZ were the dominant antibiotics in both water and sediment as their higher concentrations compared with the other compounds. Furthermore, comprehensive profiling of antibiotic resistance genes (ARGs) and microbial community was performed to gain an understanding of the evolution and dissemination of ARGs in microbial communities caused by the occurrence of antibiotics in sediment samples from Maozhou River. As a result, the sul1 gene was found to be themost abundant ARG and Proteobacteriawas the most abundantmicroorganismin all the samples (37.4-51.7%), followed by Bacteroidetes (15.3-18.4%). Statistical analysis figured out the relations among antibiotics, ARGs and microbial community. A specific conclusion could be drawn from the positive correlations among the bla_d gene, Fusobacteria, and sulfamethoxazole. It suggests that antibiotics may be positively linked to the expression of ARGs in certain bacteria, and thus high reproduction would occur within the bacterial community. Overall, the widespread distribution of ARGs underscores the need for further research on the mechanism of antibiotics influence as emerging contaminants in the environment and the associated risks to microbial community. (c) 2018 Elsevier B.V. All rights reserved.