Feeding during hibernation shifts gene expression toward active season levels in brown bears (Ursus arctos)
Feeding during hibernation shifts gene expression toward active season levels in brown bears (Ursus arctos)
复制标题
DOI：
10.1152/physiolgenomics.00030.2023
复制
发表时间：
2023-09-01
影响因子：
4.6
通讯作者：
Kelley，Joanna L.
中科院分区：
文献类型：
--
作者：
Perry，Blair W.;Mcdonald，Anna L.;Kelley，Joanna L.
熊的冬眠涉及一系列代谢和生理变化，包括胰岛素抵抗的开始，这在一定程度上是由多个组织中基因表达的全面变化所驱动的。在冬眠期间给熊喂食葡萄糖可以部分恢复活动季节的生理表型，包括对胰岛素的部分再敏化，但这种转变背后的分子机制尚不清楚。在这里，我们分析了脂肪、肝脏和肌肉组织水平的基因表达，以确定对冬眠中期葡萄糖摄食做出反应的基因，从而潜在地驱动摄食后的代谢和生理变化。我们发现，冬眠中期的摄食刺激冬眠熊的所有分析组织中的差异表达，并且这些基因的一个子集通过将表达转移到活动季节的典型水平来做出特异性反应。上游调节分子可能驱动这些进食后反应的推断涉及过氧化物酶体增殖激活受体γ (PPARG)和其他已知的胰岛素敏感性调节因子，为参与在冬眠和活跃状态之间转换代谢表型的高级调节机制提供了新的见解。
Hibernation in bears involves a suite of metabolical and physiological changes, including the onset of insulin resistance, that are driven in part by sweeping changes in gene expression in multiple tissues. Feeding bears glucose during hibernation partially restores active season physiological phenotypes, including partial resensitization to insulin, but the molecular mechanisms underlying this transition remain poorly understood. Here, we analyze tissue-level gene expression in adipose, liver, and muscle to identify genes that respond to midhibernation glucose feeding and thus potentially drive postfeeding metabolical and physiological shifts. We show that midhibernation feeding stimulates differential expression in all analyzed tissues of hibernating bears and that a subset of these genes responds specifically by shifting expression toward levels typical of the active season. Inferences of upstream regulatory molecules potentially driving these postfeeding responses implicate peroxisome proliferator-activated receptor gamma (PPARG) and other known regulators of insulin sensitivity, providing new insight into high-level regulatory mechanisms involved in shifting metabolic phenotypes between hibernation and active states.