Genomic structure and promoter activity of the E1AF gene, a member of the ETS oncogene family
Genomic structure and promoter activity of the E1AF gene, a member of the ETS oncogene family
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DOI：
10.1016/j.bbrc.2005.11.024
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发表时间：
2006-01-06
影响因子：
3.1
通讯作者：
Yoshida, K
中科院分区：
文献类型：
--
作者：
Ishida, S;Higashino, F;Yoshida, K
E1 AF是ETS癌基因家族的成员，被认为是小鼠PEA 3的人类同源物。我们已经分离了E1 AF的基因组克隆，并分析了其5 '侧翼区的启动子活性。我们确定了外显子1的变异，这取决于细胞类型。在5 '-侧翼区没有典型的TATA盒，但看到了许多转录因子的推定结合位点，包括PEA 3以及CAAT盒。荧光素酶报告基因分析表明，5 '侧翼区具有启动子活性。北方印迹研究表明E1 AF基因在限制性组织如垂体、胎盘和胎肾中显著表达。此外，E1 AF启动子被E1 AF本身和雌激素受体激活。这些发现表明E1 AF是一个管家基因，其表达在特定组织中受到控制。(c)2005年爱思唯尔公司All rights reserved.
E1AF is a member of the ETS oncogene family and is thought to be a human homologue of mouse PEA3. We have isolated a genomic clone of E1AF and analyzed the Promoter activity of its 5'-flanking region. We identified a variation in exon 1, which depends on the cell type. There was no typical TATA box in the 5'-flanking region, but putative binding sites of a number of transcription factors including PEA3 as well as CAAT boxes were seen. A luciferase reporter assay indicated that the 5'-flanking region possesses promoter activity. Northern blot studies demonstrated significant expression of the E1AF gene in restricted tissues such as the pituitary gland, placenta, and fetal kidney. Moreover, the E1AF promoter was activated by E1AF itself and estrogen receptor. These findings suggest that E1AF is a housekeeping gene, whose expression is controlled in specific tissues. (c) 2005 Elsevier Inc. All rights reserved.