Production of a monoclonal antibody reactive with human dendritic reticulum cells and its use in the immunohistological analysis of lymphoid tissue.
Production of a monoclonal antibody reactive with human dendritic reticulum cells and its use in the immunohistological analysis of lymphoid tissue.
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与人树突状网状细胞发生反应的单克隆抗体的制备及其在淋巴组织免疫组织学分析中的应用。
DOI：
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发表时间：
1983
影响因子：
3.4
通讯作者：
D. Mason
中科院分区：
文献类型：
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作者：
M. Naiem;J. Gerdes;Z. Abdulaziz;H. Stein;D. Mason
本文报道了一种能与人树突状网状细胞(DRC)发生强烈反应的鼠源性单抗(R4/23)。组织冰冻切片免疫过氧化物酶染色显示，该抗体与淋巴滤泡(原、次级淋巴滤泡)中的DRC呈强阳性反应，与边缘带脾B细胞和淋巴结皮层滤泡中的部分外周滤泡套B淋巴细胞呈弱阳性反应。抗体R4/23的价值在于它可以清楚地描绘DRC在反应性和肿瘤性淋巴组织中的分布。尤其令人感兴趣的是，所有起源于生发中心细胞的滤泡性淋巴瘤病例都始终伴有DRC的增殖，即使肿瘤出现在非淋巴组织中--例如在肾脏。相反，非生发中心起源的B细胞淋巴瘤的DRC部分或全部消失。
A murine monoclonal antibody (designated R4/23) which reacts strongly with human dendritic reticulum cells (DRC) is described. Immunoperoxidase staining of tissue cryostat sections revealed that this antibody reacts strongly with DRC in lymphoid follicles (both primary and secondary), and also weakly with marginal zone splenic B cells and with some peripheral follicular mantle B lymphocytes in lymph node cortical follicles. The value of antibody R4/23 is that it allows the distribution of DRC in reactive and neoplastic lymphoid tissue to be clearly delineated. Of particular interest is the fact that all cases of follicular lymphoma of germinal centre cell origin are consistently accompanied by a proliferation of DRC, even when the neoplasm is present in non-lymphoid tissue--for example, in the kidney. In contrast, DRC in B cell lymphomas of non-germinal centre origin are partially or totally obliterated.