N-terminal pro-C-type natriuretic peptide, but not C-type natriuretic peptide, is greatly elevated in the fetal circulation
N-terminal pro-C-type natriuretic peptide, but not C-type natriuretic peptide, is greatly elevated in the fetal circulation
复制标题
DOI：
10.1042/cs20030307
复制
发表时间：
2004-05-01
期刊：
影响因子：
6
通讯作者：
Yandle, TG
中科院分区：
文献类型：
--
作者：
Prickett, TCR;Kaaja, RJ;Yandle, TG
我们最近在人类和绵羊的血液循环中发现了一种新的肽，NT-proCNP(I-50) （n端前c型利钠肽）。先前关于免疫反应性CNP胎儿-母体梯度升高的报道提出了proCNP的加工和代谢在母体和胎儿组织中可能不同的可能性。因此，我们收集了正常妊娠和子痫前期妊娠妇女分娩时匹配的外周母体和脐带血浆样本，使用HPLC和RIA研究CNP和NT-proCNP的存在和浓度。正常脐带血浆NT-proCNP浓度比母体静脉高10倍（246 +/- 17比24.3 +/- 1.8 pmol/l, P < 0.001），远高于相应的CNP水平（胎儿和母体血浆分别为3.6±0.4比1.8±0.3 pmol/l, P < 0.001）。虽然在母体或脐带血中正常和子痫前期血浆CNP浓度没有显著差异，但NT-proCNP在源（母体或胎儿）和妊娠组（正常或子痫前期）之间显示出显著的统计学相互作用（F = 5.8, P = 0.025）。母体NT-proCNP水平在子痫前期组升高，而在脐带血中观察到相反的情况。综上所述，与母体血浆相比，胎儿血浆中NT-proCNP/CNP比值的显著升高表明胎儿组织中CNP的合成和清除率（而非NT-proCNP清除率）显著增加。
We have identified recently a new peptide, NT-proCNP(I-50) (N-terminal pro-C-type natriuretic peptide), in the circulation of humans and sheep. A previous report of an elevated fetal-maternal gradient in immunoreactive CNP raised the possibility that processing and metabolism of proCNP may differ in maternal and fetal tissues. We therefore collected matching peripheral maternal and umbilical cord plasma samples at delivery from women with normotensive and pre-eclamptic pregnancies to investigate the presence and concentrations of CNP and NT-proCNP using HPLC and RIA. Plasma concentrations of NT-proCNP in normotensive umbilical cord plasma were 10-fold higher than maternal venous levels (246 +/- 17 compared with 24.3 +/- 1.8 pmol/l; P < 0.001) and much higher than corresponding levels of CNP (3.6 ± 0.4 compared with 1.8 ± 0.3 pmol/l in the fetal and maternal plasma respectively; P < 0.001). Although there was no significant difference between normotensive and pre-eclamptic plasma CNP concentrations in either maternal or umbilical cord blood, NT-proCNP showed a significant statistical interaction (F = 5.8, P = 0.025) between the source (maternal or fetal) and gestational group (normotensive or pre-eclamptic). Maternal NT-proCNP levels were raised in the pre-eclampsia group, whereas the converse was observed in umbilical cord blood. In conclusion, the greatly elevated ratio of NT-proCNP/CNP in fetal compared with maternal plasma suggests that synthesis, as well as clearance, of CNP (but not NT-proCNP clearance) are markedly increased in fetal tissues.