Extracellular matrix with the rigidity of adipose tissue helps 3T3-L1 adipocytes maintain insulin responsiveness
Extracellular matrix with the rigidity of adipose tissue helps 3T3-L1 adipocytes maintain insulin responsiveness
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DOI：
10.2152/jmi.56.142
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发表时间：
2009-08-01
影响因子：
0.7
通讯作者：
Funaki, Makoto
中科院分区：
文献类型：
--
作者：
Li, Qinkai;Hosaka, Toshio;Funaki, Makoto
尽管3 T3-L1脂肪细胞作为体内脂肪细胞的模型系统很受欢迎，但它们并不携带体内脂肪细胞的所有细胞功能。在这项研究中，我们研究了细胞外基质（ECM）刚性对3 T3-L1脂肪细胞胰岛素信号转导的影响。在层压有1型胶原和纤连蛋白的混合物的250 Pa聚丙烯酰胺凝胶（软凝胶）上，其刚性与脂肪组织的刚性相匹配，胰岛素受体、IRS-1和AKT的表达上调，并且它们的胰岛素刺激的磷酸化增强。此外，GLUT 1的表达下调，而GLUT 4的表达不受ECM刚性降低的影响。胰岛素刺激的GLUT 4招聘质膜上的细胞接种在软凝胶显着增强。这些结果表明，调整ECM的刚性，脂肪组织增强胰岛素信号在3 T3-L1脂肪细胞和增强胰岛素刺激的GLUT 4招聘到质膜。
Despite the popularity of 3T3-L1 adipocytes as a model system of adipocytes in vivo, they do not carry all of the cellular functions of adipocytes in vivo. In this study, we investigated the effect of extracellular matrix (ECM) rigidity on insulin signal transduction in 3T3-L1 adipocytes. On 250 Pa polyacrylamide gel (soft gel) laminated with a mixture of collagen type 1 and fibronectin, whose rigidity matches that of adipose tissue, expression of the insulin receptor, IRS-1 and AKT was upregulated and their insulin - stimulated phosphorylation was enhanced. Furthermore, the expression of GLUT1 was downregulated, whereas the expression of GLUT4 was unaffected as ECM rigidity decreased. Insulin-stimulated GLUT4 recruitment to the plasma membrane was significantly enhanced in cells seeded on soft gel. These results suggest that adjusting the ECM rigidity to that of adipose tissue augments insulin signaling in 3T3-L1 adipocytes and enhances insulin-stimulated GLUT4 recruitment to the plasma membrane.