Drinking hydrogen water and intermittent hydrogen gas exposure, but not lactulose or continuous hydrogen gas exposure, prevent 6-hydorxydopamine-induced Parkinson's disease in rats.
Drinking hydrogen water and intermittent hydrogen gas exposure, but not lactulose or continuous hydrogen gas exposure, prevent 6-hydorxydopamine-induced Parkinson's disease in rats.
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DOI：
10.1186/2045-9912-2-15
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发表时间：
2012-05-20
影响因子：
2.9
通讯作者：
Ohno K
中科院分区：
文献类型：
--
作者：
Ito M;Hirayama M;Yamai K;Goto S;Ito M;Ichihara M;Ohno K
乳果糖是一种人工合成的二糖，在人类和啮齿动物体内只能被肠道细菌催化，其中大量的氢气是由细菌催化乳果糖产生的。我们之前报道了随意给氢水对预防帕金森氏病(PD)大鼠模型的显著效果。测定了28名健康受试者和37名帕金森病患者的肺泡末呼气氢浓度，以及9只大鼠服用氢水或乳果糖后的肺泡末呼气氢浓度。立体定向复制6-羟基多巴胺(6-OHDA)诱导的大鼠半脑帕金森病模型。我们比较了氢水和乳果糖预防帕金森病的效果。我们还分析了2%氢气连续和间歇给药的效果。氢气水使健康受试者的呼气氢浓度在10分钟内从8.6 ± 2.1增加到32.6 ± 3.3ppm(平均值和扫描电子显微镜，n = 8)。乳果糖增加了86%的健康受试者和59%的帕金森病患者的呼气氢浓度。与71%的健康受试者单相性氢气增加相比，32%和41%的帕金森病患者分别出现双相和无相氢气增加。乳果糖还单相增加9只大鼠的呼气氢水平。然而，乳果糖略微改善了6-OHDA诱导的大鼠帕金森病。持续使用2%的氢气同样也有轻微的效果。另一方面，间歇给予2%氢气可预防6只大鼠中的4只发生帕金森病。缺乏氢的剂量响应，以及对氢水和间歇性氢气的有利影响的存在，表明氢的信号调制活动可能在发挥对PD的保护作用中起作用。
Lactulose is a synthetic disaccharide that can be catalyzed only by intestinal bacteria in humans and rodents, and a large amount of hydrogen is produced by bacterial catalysis of lactulose. We previously reported marked effects of ad libitum administration of hydrogen water on prevention of a rat model of Parkinson’s disease (PD). End-alveolar breath hydrogen concentrations were measured in 28 healthy subjects and 37 PD patients, as well as in 9 rats after taking hydrogen water or lactulose. Six-hydroxydopamine (6-OHDA)-induced hemi-PD model was stereotactically generated in rats. We compared effects of hydrogen water and lactulose on prevention of PD. We also analyzed effects of continuous and intermittent administration of 2% hydrogen gas. Hydrogen water increased breath hydrogen concentrations from 8.6 ± 2.1 to 32.6 ± 3.3 ppm (mean and SEM, n = 8) in 10 min in healthy subjects. Lactulose increased breath hydrogen concentrations in 86% of healthy subjects and 59% of PD patients. Compared to monophasic hydrogen increases in 71% of healthy subjects, 32% and 41% of PD patients showed biphasic and no increases, respectively. Lactulose also increased breath hydrogen levels monophasically in 9 rats. Lactulose, however, marginally ameliorated 6-OHDA-induced PD in rats. Continuous administration of 2% hydrogen gas similarly had marginal effects. On the other hand, intermittent administration of 2% hydrogen gas prevented PD in 4 of 6 rats. Lack of dose responses of hydrogen and the presence of favorable effects with hydrogen water and intermittent hydrogen gas suggest that signal modulating activities of hydrogen are likely to be instrumental in exerting a protective effect against PD.