Prevalence of group A genotype human rotavirus among children with diarrhoea in Nepal, 2009-2011.
Prevalence of group A genotype human rotavirus among children with diarrhoea in Nepal, 2009-2011.
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DOI：
10.4103/2224-3151.207045
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发表时间：
2012-10-01
影响因子：
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通讯作者：
Mahasethd, Chandeshwar
中科院分区：
文献类型：
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作者：
Sherchand, Jeevan B;Schluter, W William;Mahasethd, Chandeshwar
背景：已知轮状病毒作为儿童腹泻的病原体在5岁以下儿童中引起严重疾病。方法：2009 - 2011年对2718份粪便标本采用酶免疫法检测轮状病毒，同时采用ELISA和RT-PCR法检测A组轮状病毒。结果：轮状病毒在住院患者中检出率（28.5%）高于门诊患者（15.2%）。在三年的研究期间，653例（24.4%）轮状病毒ELISA阳性。对638份样本进行RT-PCR基因分型。最常见的基因型是G12 P [6]（60.4%）。混合感染并不罕见（2009年为14%，2010年为29%，2011年为7%）。然而，在研究期间，41例进行了部分分型，23例完全未分型。结论：本研究强调了尼泊尔流行的轮状病毒疾病负担和轮状病毒株的多样性。持续的哨点监测将为决策者提供关于轮状病毒疫苗引进的有用信息。
BACKGROUND: Rotavirus as a causative agent of childhood diarrhea is known to cause serious illness among children less than 5 years of age. This study examined the epidemiology of rotavirus disease burden and diversity of G and P genotypes of rotavirus in Nepal.METHODS: Stool samples were tested for rotavirus by Enzyme Immuno Assay and Group A rotaviruses were detected by using both ELISA and RT-PCR in 2718 samples between 2009 and 2011.RESULTS: Rotavirus was more frequently detected among inpatients (28.5%) than outpatients (15.2%). Over the three-year study period, 653 (24.4%) cases were positive for rotavirus by ELISA. Genotyping by RT-PCR was done on 638 samples. The most prevalent genotype was G12P [6] (60.4%). Mixed infections were not uncommon (14% in 2009, 29% in 2010 an 7% in 2011). However, 41 were partially typed and 23 were completely untyped over the study period.CONCLUSIONS: This study highlights the rotavirus disease burden and diversity of rotavirus strains circulating in Nepal. Continued sentinel surveillance will provide useful information to policy makers with regard to rotavirus vaccine introduction.