Ambient Ionization Mass Spectrometry for Cancer Diagnosis and Surgical Margin Evaluation.
Ambient Ionization Mass Spectrometry for Cancer Diagnosis and Surgical Margin Evaluation.
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DOI：
10.1373/clinchem.2014.237172
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发表时间：
2016-01
影响因子：
9.3
通讯作者：
Eberlin LS
中科院分区：
文献类型：
--
作者：
Ifa DR;Eberlin LS
临床上需要能够基于诊断性分子特征实现疾病快速诊断的新技术。环境电离质谱技术彻底改变了从组织样本中实时获取分子信息且只需极少样本预处理的方式。应用于临床研究的环境电离技术的新进展表明，环境电离质谱很快将成为组织诊断的常规医疗工具。
本综述总结了应用于组织分析的环境电离技术的主要进展，重点关注解吸电喷雾电离质谱、探针电喷雾电离、接触喷雾和快速蒸发电离质谱。我们描述了它们在人类癌症研究和手术切缘评估中的应用，强调了用于体外和体内人类癌症组织分析的综合方法。我们还讨论了这些工具在临床应用中面临的挑战，并对该领域的未来提出了展望。
多项研究已经展示了环境电离质谱在快速、准确诊断癌症方面的价值。小分子已被确定为潜在的诊断生物标志物，包括代谢物、脂肪酸和甘油磷脂。统计分析能够以较高的准确率（>95%）对组织进行区分，这是很常见的情况。这个新兴领域在被广泛接受为癌症诊断的医疗工具之前，还有一些挑战需要克服。对新型综合环境电离质谱技术的研究不断增加，现有工具也在持续改进，这使得该领域在未来转化应用于临床方面非常有前景。
There is a clinical need for new technologies that would enable rapid disease diagnosis based on diagnostic molecular signatures. Ambient ionization mass spectrometry has revolutionized the means by which molecular information can be obtained from tissue samples in real time and with minimal sample pretreatment. New developments in ambient ionization techniques applied to clinical research suggest that ambient ionization mass spectrometry will soon become a routine medical tool for tissue diagnosis. This review summarizes the main developments in ambient ionization techniques applied to tissue analysis, with focus on desorption electrospray ionization mass spectrometry, probe electrospray ionization, touch spray, and rapid evaporative ionization mass spectrometry. We describe their applications to human cancer research and surgical margin evaluation, highlighting integrated approaches tested for ex vivo and in vivo human cancer tissue analysis. We also discuss the challenges for clinical implementation of these tools and offer perspectives on the future of the field. A variety of studies have showcased the value of ambient ionization mass spectrometry for rapid and accurate cancer diagnosis. Small molecules have been identified as potential diagnostic biomarkers, including metabolites, fatty acids, and glycerophospholipids. Statistical analysis allows tissue discrimination with high accuracy rates (>95%) being common. This young field has challenges to overcome before it is ready to be broadly accepted as a medical tool for cancer diagnosis. Growing research in new, integrated ambient ionization mass spectrometry technologies and the ongoing improvements in the existing tools make this field very promising for future translation into the clinic.