Accurate determination of microbial diversity from 454 pyrosequencing data
Accurate determination of microbial diversity from 454 pyrosequencing data
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DOI：
10.1038/nmeth.1361
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发表时间：
2009-09-01
期刊：
影响因子：
48
通讯作者：
Sloan, William T.
中科院分区：
文献类型：
--
作者：
Quince, Christopher;Lanzen, Anders;Sloan, William T.
我们提出了一种算法，PyroNoise，聚类的454焦磷酸测序读取使用的距离测量模型的测序噪声的流程图。这推断了扩增子集合中的真实序列。我们对从湖泊中提取的微生物16S rDNA序列的已知混合物进行了pyrosequenced，发现如果没有降噪，操作分类单元的数量被高估，但使用PyroNoise可以准确计算。
We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.