Effect of Long-Term Exercise Training on lncRNAs Expression in the Vascular Injury of Insulin Resistance
Effect of Long-Term Exercise Training on lncRNAs Expression in the Vascular Injury of Insulin Resistance
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长期运动训练对胰岛素抵抗血管损伤中lncRNAs表达的影响
DOI：
10.1007/s12265-018-9830-0
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发表时间：
2018-12-01
影响因子：
3.4
通讯作者：
Dun, Yaoshan
中科院分区：
文献类型：
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作者：
Liu, Suixin;Zheng, Fan;Dun, Yaoshan
利用LNCRNA微阵列分析来研究胰岛素抵抗中主动脉内皮中LNCRNA和mRNA的全球差异表达的运动影响。将24名男性C57BL/6J小鼠随机分为三组：对照组（n = 8），高脂饮食组（n = 8）和高脂饮食加上运动训练组（n = 8）。使用LNCRNA微阵列分析来研究三组主动脉内皮中LNCRNA和mRNA的全局差异表达，并通过qPCR进一步验证了结果。应用高几何分布分析以揭示可能的信号传导。运动可能减轻IR通过FR030200-COL3A1，FR402720-RND1和FR030200/FR402720-RND3信号在细胞骨架重排途径中的血管损伤； E2F1-FR030200/ FR402720-NNAT和FR030200/ FR402720-FAM46A抗炎途径中的信号。这项研究确定了一组失调的LNCRNA和mRNA，可能是与高脂饮食引起的胰岛素抵抗性血管损伤相关的潜在生物标志物。
LncRNA microarray analysis was applied to investigate the exercise effect on the global differential expressions of lncRNA and mRNA in aorta endothelium in insulin resistance. Twenty-four male C57BL/6 J mice were randomly divided into three groups: control group (n= 8), high-fat diet group (n= 8), and high-fat diet plus exercise training group (n= 8). An lncRNA microarray analysis was applied to investigate the global differential expressions of lncRNA and mRNA in aorta endothelium among three groups and the results were further verified by qPCR. Hypergeometric distribution analysis was applied to reveal the possible signaling. Exercise might alleviate the vascular injury of IR viaFR030200-Col3A1,FR402720-Rnd1, andFR030200/FR402720-Rnd3signalings in cytoskeletal rearrangement pathway;E2F1-FR030200/FR402720-NnatandFR030200/ FR402720-Fam46asignalings in anti-inflammation pathway. This study identified a panel of dysregulated lncRNAs and mRNAs that might serve as potential biomarkers relevant to the vascular injury in insulin resistance induced by high-fat diet.