ON THE ACCESS OF BLOOD-BORNE DYES TO VARIOUS TUMOUR REGIONS
ON THE ACCESS OF BLOOD-BORNE DYES TO VARIOUS TUMOUR REGIONS
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DOI：
10.1038/bjc.1962.36
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发表时间：
1962-01-01
影响因子：
8.8
通讯作者：
SYLVEN, B
中科院分区：
文献类型：
--
作者：
GOLDACRE, RJ;SYLVEN, B
通过用无害的染料改变活体动物体内血液的颜色，特别是用李萨明绿，就有可能标出血液传播物质不易到达的肿瘤区域。这些区域突然出现在肿瘤中，在一个临界年龄，在所研究的移植小鼠肿瘤(乳腺癌、肉瘤37、Ehrlich-Landschiitz固体腹水)中约为12天，而在大鼠Walker癌肉瘤中约为5-10天，具体取决于宿主的类型。几天后，这些区域通常占据肿瘤的所有区域，除了一个血管良好的薄壳，厚度从几毫米到十分之一毫米不等。当身体其他部位的血液为绿色时，它们通常含有红色血液的血管，表明血管被堵塞。这些未着色的区域含有活细胞，如生命染色、组织培养，特别是通过移植到新的宿主中。在来自这些白色区域的41例移植中，产生了23例典型肿瘤，包括所有测试的肿瘤类型。在血液变绿后的不同时间间隔，以及在每天重复注射染料和注射到坏死区之后，测定染料对这些包含活细胞的所谓肿瘤坏死区的渗透。在任何情况下，在整个坏死区都不能达到高浓度。间质液体的运动可能会将染料带入肿瘤内部，但在有坏死中心的肿瘤中，间质液体的运动被延缓或阻止，尽管每个肿瘤在这方面都有各自的表现。讨论了这些发现在肿瘤掺入研究、假定的肿瘤染料“浓度”、肿瘤生化数据的解释和抗肿瘤药物的作用方面的一般意义。
By changing the color of systemic blood in the living animal with harmless dyes, and especially with lissamine green, it is possible to mark out regions of tumors which cannot readily be reached by blood-borne substances. These regions suddenly appeared in tumors, at a critical age, which in the transplanted mouse tumors studied (mammary carcinoma, sarcoma 37, Ehrlich-Landschiitz solid ascites) is about 12 days, and in the rat Walker carcinosarcoma about 5-10 days, depending on the type of host. A few days later these zones usually occupied all but a thin well-vascularized shell of the tumor, varying from a few millimetres in thickness to as little as one tenth of a millimetre. They often contained vessels with red blood in them when the blood in the rest of the body is green, showing that the vessels are blocked. These uncolored zones contained living cells, as shown by vital staining, tissue culture and particularly, by transplantation into new hosts. In 41 transplantations from these "white" zones, 23 typical tumors are produced, including all the tumor types tested. The penetration of dye into these so-called necrotic zones of tumors which contain living cells is determined at various time intervals after the blood was made green, and also after repeated daily injections of dye, and injections into the necrotic zone. In no case is a high concentration achieved throughout the necrotic zone. The movement of interstitial fluid, which might have carried the dye into the interior of the tumor, is retarded or prevented in tumors with necrotic centres, although each tumor behaved individually in this respect. The general significance of these findings is discussed in relation to tumor incorporation studies, the supposed "concentration" of dyes of tumors, the interpretation of tumor biochemical data and the action of anti-tumor agents.