Analysis and HPLC fractionation of urine from patients with cystic fibrosis, chronic lung diseases and normal controls.
Analysis and HPLC fractionation of urine from patients with cystic fibrosis, chronic lung diseases and normal controls.
复制标题
对囊性纤维化、慢性肺病和正常对照患者的尿液进行分析和 HPLC 分离。
DOI：
10.1016/s0009-9120(89)80036-0
复制
发表时间：
1989
影响因子：
2.8
通讯作者：
Alhadeff,JA
中科院分区：
文献类型：
--
作者：
Guman-Wignot,TM;Kaufman,J;HolsclawJr,DS;Schmoyer,IR;Alhadeff,JA
测定了19例囊性纤维化(CF)患者、12例正常对照和11例慢性肺部疾病病理对照的尿肌酐、蛋白质、碳水化合物和唾液酸的含量。与正常对照组相比，总CF组和CF亚组的平均肌酐排泄水平显着降低，但与病理对照组相当。与正常对照组和病理对照组相比，CF患者的平均尿蛋白水平似乎升高，但增加的水平是由CF以外的因素(如糖尿病的存在)引起的。与正常对照组和病理对照组相比，非糖尿病CF患者组的尿液和尿液中总碳水化合物和唾液酸量无显著差异。Low M，(<；10,000 Dalton)尿标本的高效液相色谱分析显示，在所有接受抗生素治疗的CF患者中，43%的CF患者服用低剂量药物或不服用药物，17%的正常对照组和9%的病理对照组存在一个未知的峰。目前的结果说明了包括适当的病理对照和根据临床因素和所采用的治疗类型将患者分为亚组的重要性。
The amounts of creatinine, protein, carbohydrate and sialic acid in the urine of 19 patients with cystic fibrosis (CF), 12 normal controls and 11 pathological controls with chronic lung disease have been determined. The mean creatinine excretion levels of the total CF group as well as the CF subgroups are significantly decreased when compared to normal controls but comparable to pathological controls. Mean urinary protein levels appear to be increased in patients with CF compared to normal controls and pathological controls but the increased levels resulted from factors (e.g.presence of diabetes mellitus) other than CF. No significant differences were found in amounts of total carbohydrate and sialic acid in urine and fractionated urinary preparations for the total group of nondiabetic patients with CF when compared to both normal and pathological controls. HPLC fractionation of lowM, (<10,000 Daltons) urinary preparations indicated the presence of an unknown peak in all of the antibiotic-treated CF patients, 43% of CF patients on low or no medication, 17% of the normal controls and 9% of the pathological controls. The present results illustrate the importance of including appropriate pathological controls and dividing patients with CF into subgroups according to clinical factors and types of therapy employed.