A parallel microfluidic channel fixture fabricated using laser ablated plastic laminates for electrochemical and chemiluminescent biodetection of DNA
A parallel microfluidic channel fixture fabricated using laser ablated plastic laminates for electrochemical and chemiluminescent biodetection of DNA
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DOI：
10.1063/1.3664694
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发表时间：
2011-12-01
期刊：
影响因子：
3.2
通讯作者：
Brozik, Susan M.
中科院分区：
文献类型：
--
作者：
Edwards, Thayne L.;Harper, Jason C.;Brozik, Susan M.
本文描述了一种塑料多层三通道微流控夹具的制造和使用。采用激光烧蚀方法对塑料聚甲基丙烯酸甲酯和聚对苯二甲酸乙二酯层压板进行激光加工，制备了多层器件。该装置由九个可单独寻址的金或金/ITO工作电极阵列和一个电阻铂加热元件组成。激光加工塑料层压板中的流体通道，以及用于热蒸发以在塑料层压板上形成电极区域的模板掩模，使设计更改能够快速且廉价地实施。通过ITO电极实现并监测了夹具中的电致化学发光反应。采用可电寻址芳基重氮化学方法选择性地形成金电极，用于双链DNA(DsDNA)样品中多分析物DNA的电化学检测。DsDNA的电化学检测是通过在集成加热器的作用下将dsDNA分子熔化在溶液中实现的，从而允许通过非特异性结合对照来检测乳腺癌和结直肠癌的特异性DNA序列。在检测之后，可以使用集成加热元件通过高温(95摄氏度)剥离来更新阵列表面。这种通用而简单的设备原型制作方法显示了进一步开发高度集成的多功能生物分析设备的潜力。(C)2011年美国物理研究所。[DOI：10.1063/1.3664694]
Herein is described the fabrication and use of a plastic multilayer 3-channel microfluidic fixture. Multilayer devices were produced by laser machining of plastic polymethylmethacrylate and polyethyleneterapthalate laminates by ablation. The fixture consisted of an array of nine individually addressable gold or gold/ITO working electrodes, and a resistive platinum heating element. Laser machining of both the fluidic pathways in the plastic laminates, and the stencil masks used for thermal evaporation to form electrode regions on the plastic laminates, enabled rapid and inexpensive implementation of design changes. Electrochemiluminescence reactions in the fixture were achieved and monitored through ITO electrodes. Electroaddressable aryl diazonium chemistry was employed to selectively pattern gold electrodes for electrochemical multianalyte DNA detection from double stranded DNA (dsDNA) samples. Electrochemical detection of dsDNA was achieved by melting of dsDNA molecules in solution with the integrated heater, allowing detection of DNA sequences specific to breast and colorectal cancers with a non-specific binding control. Following detection, the array surface could be renewed via high temperature (95 degrees C) stripping using the integrated heating element. This versatile and simple method for prototyping devices shows potential for further development of highly integrated, multi-functional bioanalytical devices. (C) 2011 American Institute of Physics. [doi:10.1063/1.3664694]