Effects of Exogenous Trehalose on the Metabolism of Sugar and Abscisic Acid in Tomato Seedlings Under Salt Stress
Effects of Exogenous Trehalose on the Metabolism of Sugar and Abscisic Acid in Tomato Seedlings Under Salt Stress
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DOI：
10.1007/s12209-019-00214-x
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发表时间：
2019-07
影响因子：
7.1
通讯作者：
Yanchun Feng;Xiuyu Chen;Yulong He;X. Kou;Zhaohui Xue
中科院分区：
文献类型：
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作者：
Yanchun Feng;Xiuyu Chen;Yulong He;X. Kou;Zhaohui Xue
盐胁迫影响植物的生长发育，导致作物品质和产量下降。本研究以番茄幼苗为材料，采用盐和海藻糖处理，通过与理想溶液相似性排序分析，筛选出最佳海藻糖处理浓度。同时测定了糖和脱落酸（阿坝）的含量，并通过实时荧光定量PCR检测了糖和阿坝代谢相关基因的表达。结果表明，盐胁迫下番茄幼苗的最适海藻糖浓度为2 mmol/L。外源海藻糖通过影响淀粉和可溶性糖代谢相关基因的表达，降低淀粉含量，提高可溶性糖含量。外源海藻糖通过诱导糖转运蛋白基因的上调而改变了糖的积累和分布。此外，海藻糖通过调控阿坝合成代谢相关基因的表达，提高阿坝含量，诱导盐胁迫反应。海藻糖能有效缓解盐胁迫，提高番茄的耐盐性。这些结果为番茄耐盐机制的研究提供了新的视角和资源，也为缓解番茄盐胁迫提供了新的途径。
Salt stress affects the growth and development of plants, which results in a decrease in crop quality and yield. In this study, we used tomato seedlings treated with salt and trehalose as experimental materials and analyzed them using the technique for order preference by similarity to ideal solution analysis to select the optimal trehalose concentration for treatment. We also determined the contents of sugar and abscisic acid (ABA) and detected the expression of genes involved in the metabolism of sugar and ABA by quantitative real-time PCR. Results showed that the optimal trehalose concentration was 2 mmol/L for tomato seedlings under salt stress. Exogenous trehalose decreased the starch content and increased the soluble sugar content by affecting the expression of genes related to the metabolism of starch and soluble sugar. Exogenous trehalose altered the accumulation and distribution of sugar by inducing the upregulation of sugar transporter genes. Furthermore, trehalose increased the ABA content to induce salt stress response by regulating the expression of genes related to the synthesis and metabolism of ABA. In conclusion, trehalose can effectively alleviate salt stress and enhance salt tolerance of tomato. These findings provide a novel perspective and a better resource to investigate the salt tolerance mechanism and a new method for alleviating salt stress in tomato.