Zebrafish complement factor H and its related genes: identification, evolution, and expression
Zebrafish complement factor H and its related genes: identification, evolution, and expression
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DOI：
10.1007/s10142-010-0182-3
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发表时间：
2010-07
影响因子：
2.9
通讯作者：
G. Sun;Hongyan Li;Y. Wang;Baohong Zhang;Shicui Zhang
中科院分区：
文献类型：
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作者：
G. Sun;Hongyan Li;Y. Wang;Baohong Zhang;Shicui Zhang
补体系统由许多血清和膜结合蛋白组成，这些蛋白在保护宿主机体免受微生物感染方面发挥着至关重要的作用。补体因子H(CFH)调节血浆中补体的替代途径，并介导细胞表面对替代途径激活剂和非激活剂的区分。虽然斑马鱼的补体系统已经得到了广泛的研究，但关于CFH及其相关基因在这个重要的模式物种中的信息仍然很少。在本研究中，我们报道了丹尼欧CFHL1-4(CFHL1-4)基因的克隆和鉴定。序列比较和系统发育、共线以及基因组结构分析表明，CFHandCFHLS区域的骨架在从骨鱼到人类的进化过程中是保守的，CFHandCFHL1-4起源于22号染色体上的染色体内重复。此外，实时定量聚合酶链式反应显示，这两种斑马鱼CFHandCFHL主要在肝脏中以组织特异性的方式表达，并且它们的表达可以被免疫反应的诱导剂脂多糖诱导，这表明它们可能参与了急性时相反应。这些数据在硬骨鱼中尚属首次，为进一步研究它们的生理功能奠定了基础。
The complement system consists of a number of serum and membrane-bound proteins that play a crucial role in protecting the host organism against microbial infection. Complement factor H (CFH) regulates the alternative pathway of complement in plasma and mediates discrimination of cellular surfaces to alternative pathway activators and non-activators. Although complement system of zebrafish has been extensively studied, the information regardingCFHand its related genes in this important model species remains lacking. In this study, we report the molecular cloning and identification ofCFHandcomplement factor H-like 1–4(CFHL1–4) inDanio rerio. Sequence comparison and phylogenetic, syntenic, as well as genomic structure analyses demonstrated that the scaffold encompassingCFHandCFHLs region was conserved during evolution from bony fish to humans, andCFHandCFHL1–4originated by intra-chromosome duplication on chromosome 22. Besides, quantitative real-time polymerase chain reaction revealed that both zebrafishCFHandCFHLs were predominantly expressed in the liver in a tissue-specific manner, and their expression was inducible by lipopolysaccharide, an inducer of immune responses, suggesting that they are possibly involved in acute phase responses. These are the first such data in bony fish, laying a foundation for further study of their physiological functions.