RNA recognition and translational regulation by a homeodomain protein
RNA recognition and translational regulation by a homeodomain protein
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DOI：
10.1038/379694a0
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发表时间：
1996-02-22
期刊：
影响因子：
64.8
通讯作者：
Struhl, G
中科院分区：
文献类型：
--
作者：
Dubnau, J;Struhl, G
在果蝇中，前模式的主要决定因素是梯度形态原bicoid（bcd），一种结合DNA并在不同阈值浓度下转录激活靶基因的同源结构域蛋白。在这里，我们提出的证据表明，bcd也结合RNA，并作为一个翻译抑制剂，以产生一个相反的梯度的同源结构域蛋白尾（CAD）。bcd与RNA的结合似乎涉及bcd同源结构域与cad信使RNA 3'非翻译区内的离散靶序列之间的直接相互作用，并阻断cad翻译的起始。
In Drosophila, the primary determinant of anterior pattern is the gradient morphogen bicoid (bcd), a homeodomain protein that binds DNA and transcriptionally activates target genes at different threshold concentrations. Here we present evidence that bcd also binds RNA and acts as a translational repressor to generate an opposing gradient of the homeodomain protein caudal (cad). RNA binding by bcd seems to involve direct interactions between the bcd homeodomain and discrete target sequences within the 3' untranslated region of the cad messenger RNA and to block the initiation of cad translation.