Cloning, sequencing, expression and function of a cDNA encoding a receptor for the opioid growth factor, [Met(5)]enkephalin.
Cloning, sequencing, expression and function of a cDNA encoding a receptor for the opioid growth factor, [Met(5)]enkephalin.
复制标题
编码阿片类生长因子[Met(5)]脑啡肽受体的 cDNA 的克隆、测序、表达和功能。
DOI：
10.1016/s0006-8993(99)02046-6
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发表时间：
1999
期刊：
影响因子：
2.9
通讯作者：
McLaughlin,PJ
中科院分区：
文献类型：
--
作者：
Zagon,IS;Verderame,MF;Allen,SS;McLaughlin,PJ
天然阿片样物质生长因子（OGF），[Met 5]脑啡肽，是一种在发育、癌症、细胞更新、伤口愈合和血管生成期间调节细胞增殖和组织组织形成的滋补抑制肽。OGF的作用是由受体机制介导的。我们已经克隆并测序了OGF受体（OGFr）的2.1kb cDNA。发现开放阅读框架编码580个氨基酸的蛋白质，并且每个9个氨基酸的8个不完全重复是显著特征。该cDNA编码的蛋白质具有OGFr的药理学、时间和空间特性。使用反义技术的功能研究证明了细胞生长的增强。OGFr的分子结构与经典阿片受体没有同源性。这些结果为OGF和OGFr在生长过程中的相互作用提供了分子有效性。
The native opioid growth factor (OGF), [Met5]enkephalin, is a tonic inhibitory peptide that modulates cell proliferation and tissue organization during development, cancer, cellular renewal, wound healing and angiogenesis. OGF action is mediated by a receptor mechanism. We have cloned and sequenced a 2.1-kilobase (kb) cDNA for a receptor to OGF (OGFr). The open reading frame was found to encode a protein of 580 amino acids, and eight imperfect repeats of nine amino acids each were a prominent feature. The protein encoded by this cDNA exhibited the pharmacological, temporal and spatial characteristics of the OGFr. Functional studies using antisense technology demonstrated an enhancement in cell growth. The molecular organization of the OGFr has no homology to classical opioid receptors. These results provide molecular validity for the interaction of OGF and OGFr in the regulation of growth processes.