Molecular genetic analysis among subspecies of two Eurasian sturgeon species, Acipenser baerii and A-stellatus
Molecular genetic analysis among subspecies of two Eurasian sturgeon species, Acipenser baerii and A-stellatus
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DOI：
10.1046/j.1365-294x.1999.00816.x
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发表时间：
1999-12-01
影响因子：
4.9
通讯作者：
Desalle, R
中科院分区：
文献类型：
--
作者：
Doukakis, P;Birstein, VJ;Desalle, R
西伯利亚鲟（Acipenser baerii）和星鲟（A.利用线粒体DNA（mtDNA）（D-loop、细胞色素B（cyt-B）和ND5/6基因）测序研究了stellatus，以确定传统定义的亚种是否对应于分类实体和保护管理单位。最初，每个分类群有几个mtDNA区域（A. baerii：737 bp D-loop、750 bp ND 5、200 bp ND 6和790 bp cyt-b; A. stellatus：737 bp D-loop和600 bp ND5）。D环是最可变的区域，并测序35 A。baerii和82 A. stellatus个体。在任何亚种之间均未发现固定的诊断差异。单倍型的地理结构在A. baerii，基因流估计表明A. baerii baicalensis亚种以及Yenisie和Lena River种群。在A的遗传数据中没有发现种内的细分。星状的使用的系统发育标准（固定的诊断差异），用于确定保护单位进行比较的理由和其他方法的结果。总体而言，基于形态和地理的鲟科亚种命名可能不直接对应于适合管理的生物实体，不应在没有遗传支持的情况下用于保护计划。
Two species, the Siberian sturgeon, Acipenser baerii, and stellate sturgeon, A. stellatus, were studied using mitochondrial DNA (mtDNA) (D-loop, cytochrome b (cyt-b) and ND5/6 genes) sequencing to determine whether traditionally defined subspecies correspond to taxonomic entities and conservation management units. Initially several mtDNA regions for each taxon (A. baerii: 737 bp D-loop, 750 bp ND5, 200 bp ND6, and 790 bp cyt-b; A. stellatus: 737 bp D-loop and 600 bp ND5) were examined. The D-loop was the most variable region and was sequenced for 35 A. baerii and 82 A. stellatus individuals. No fixed, diagnostic differences were found between any of the subspecies. Geographical structuring of haplotypes was observed within A. baerii, and gene flow estimates suggest isolation of the A. baerii baicalensis subspecies and the Yenisie and Lena River populations. No intraspecific subdivisioning was found within the genetic data for A. stellatus. The use of the phylogenetic criterion (fixed diagnostic differences) for identifying conservation units is compared to the rationale and results of other methods. Overall, morphologically and geographically based subspecies designations within Acipenseridae may not directly correspond to the biological entities appropriate for management and should not be used far conservation programmes without genetic support.