Distribution and Diversity of Ocular Microbial Communities in Diabetic Patients Compared with Healthy Subjects
Distribution and Diversity of Ocular Microbial Communities in Diabetic Patients Compared with Healthy Subjects
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DOI：
10.1080/02713683.2017.1406528
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发表时间：
2018-01-01
影响因子：
2
通讯作者：
Kwon, Man Jae
中科院分区：
文献类型：
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作者：
Ham, Baknoon;Hwang, Hyung Bin;Kwon, Man Jae
目的：本研究的目的是确定在主要（核心与变量）微生物属的人类受试者与非diabetes.Methods的差异：从结膜拭子的19名健康受试者和30名糖尿病患者获得的细菌16S rRNA基因测序使用Illumina MiSeq平台，并使用QIIME 1.9.1测序数据进行分析。结果：多样性指数和稀疏度分析表明，糖尿病患者眼表微生物多样性高于健康人眼表微生物多样性。变形菌门、厚壁菌门、放线菌门、蓝细菌门和拟杆菌门是OS中的优势类群，糖尿病患者和对照组之间细菌门的相对丰度存在显著差异。变形菌在糖尿病组中更丰富，而厚壁菌门在对照组中更丰富。在属水平上的细菌分类群的分析表明，糖尿病患者的核心微生物组包括不动杆菌，伯克霍尔德氏菌，鞘氨醇单胞菌属，和罗尔斯通氏菌，而对照组包括慢生根瘤菌科，葡萄球菌，棒状杆菌，假单胞菌，新鞘氨醇菌，奈瑟氏菌科，和不动杆菌。结论：糖尿病患者和健康受试者之间的微生物群落组成有显着差异。与其他器官表面相比，糖尿病患者OS中不动杆菌的高丰度可能是由于OS的独特特征所致。
Purpose: The aim of this study was to identify differences in the major (core vs. variable) microbial genera of human subjects with and without diabetes.Methods: Bacterial 16S rRNA genes obtained from conjunctival swabs of 19 healthy subjects and 30 diabetic patients were sequenced using the Illumina MiSeq platform, and the sequencing data were analyzed using QIIME 1.9.1. To elucidate the microbial diversity in the ocular surface (OS), test programs from various bioinformatics domains were used.Results: Diversity index and rarefaction analysis showed that the microbial community of the diabetic patients was more diverse than that of the healthy subjects. Proteobacteria, Firmicutes, Actinobacteria, Cyanobacteria and Bacteroidetes were the dominant taxa present in the OS, and there was a significant difference in the relative abundance of the bacterial phyla between the diabetic patients and control subjects. Proteobacteria were more abundant in the diabetic group, whereas Firmicutes was more abundant in the control group. Analysis of bacterial taxa at the genus level showed that the core microbiome of diabetic patients comprised Acinetobacter, Burkholderia, Sphingomonas, and Ralstonia, whereas that of the controls comprised Bradyrhizobiaceae, Staphylococcus, Corynebacterium, Pseudomonas, Novosphingobium, Neisseriaceae, and Acinetobacter.Conclusions: There was a significant difference in the microbial community composition between diabetic patients and healthy subjects. A high abundance of Acinetobacter in the OS of diabetic patients may arise from the unique characteristics of the OS compared with those of other organ surfaces.