Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel
Confocal micro-PIV measurements of three-dimensional profiles of cell suspension flow in a square microchannel
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DOI：
10.1088/0957-0233/17/4/026
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发表时间：
2006-04-01
影响因子：
2.4
通讯作者：
Yamaguchi, Takami
中科院分区：
文献类型：
--
作者：
Lima, Rui;Wada, Shigeo;Yamaguchi, Takami
为了更好地理解微循环的生物力学，对体外微循环中血流速度分布的详细测量是非常重要的。因此，高精度、高空间分辨率地确定悬浮血细胞对流动行为的影响是非常重要的。我们使用共聚焦粒子图像测速系统(PIV)测量了方形微通道内生理液体中悬浮的血细胞的流量，并与纯水进行了比较。这项新兴技术将传统的PIV系统与旋转共焦显微镜相结合，能够获得高分辨率图像和三维(3D)光学截面速度测量。实验结果与理论计算结果吻合较好，表明大尺度流动理论可用于预测均质流体在100am方形微通道内的流动行为。我们的结果也证明了共聚焦系统的潜力，可以生成3D轮廓，从而获得使用均匀和非均匀流体(如血细胞悬浮液)的微通道中微尺度效应的详细信息。此外，从我们的共焦Micro-PIV系统获得的结果表明，该系统能够测量血液细胞悬浮液中高达0.52 mm S(-1)的速度。
A detailed measurement of the blood flow velocity profile in microchannels in vitro is fundamental to better understand the biomechanics of microcirculation. Therefore it is very important to determine the influence of suspended blood cells on the flow behaviour with high accuracy and spatial resolution. We measured the flow of blood cells suspended in a physiological fluid within a square microchannel using a confocal particle image velocimetry (PIV) system and compared it to pure water. This emerging technology combines a conventional PIV system with a spinning confocal microscope and has the ability to obtain high-resolution images and three-dimensional (3D) optical section velocity measurements. The good agreement obtained between the measured and estimated results suggests that macroscale flow theory can be used to predict the flow behaviour of a homogeneous fluid within a 100 Am square microchannel. Our results also demonstrated the potential of the confocal system for generating 3D profiles and consequently obtaining detailed information on microscale effects in microchannels using both homogeneous and non-homogeneous fluids, such as a suspension of blood cells. Furthermore, the results obtained from our confocal micro-PIV system show the ability of this system to measure velocities up to 0.52 mm s(-1) in a blood cell suspension fluid.