Silver-intensified gold and peroxidase as dual ultrastructural immunolabels for pre- and postsynaptic neurotransmitters.
Silver-intensified gold and peroxidase as dual ultrastructural immunolabels for pre- and postsynaptic neurotransmitters.
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银强化金和过氧化物酶作为突触前和突触后神经递质的双重超微结构免疫标记。
DOI：
10.1126/science.2858916
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
vandenPol,AN
中科院分区：
文献类型：
--
作者：
vandenPol,AN
一个超微结构的免疫染色方法，使用银强化金与另一个程序相结合，使用生物素过氧化物酶共轭物，允许同时识别两个神经递质相关抗原在中枢神经系统。酪氨酸羟化酶免疫反应阳性神经元标记的银强化金可以区分在光镜和电镜水平的谷氨酸脱羧酶免疫反应阳性神经元标记的过氧化物酶。第二组免疫试剂与第一组的交叉反应性被胶体金免疫球蛋白复合物周围形成的重金属银壳降低。用这种双重预包埋方法，发现含有抑制性神经递质γ-氨基丁酸的过氧化物酶染色轴突直接与大鼠下丘脑背内侧的银染多巴胺神经元形成突触。这种方法可以与包埋后免疫细胞化学胶体金程序结合使用，允许在同一组织切片中对三种神经递质相关抗原进行超微结构鉴定。
An ultrastructural immunostaining method that uses silver-intensified gold was combined with another procedure that uses biotin peroxidase conjugates to allow simultaneous identification of two neurotransmitter-related antigens in the central nervous system. Tyrosine hydroxylase-immunoreactive neurons labeled with silver-intensified gold could be differentiated at both light and electron microscopic levels from glutamate decarboxylase-immunoreactive neurons labeled with peroxidase. Cross reactivity of the second group of immunoreagents with the first group was reduced by the heavy metal silver shell formed around the colloidal gold immunoglobulin complex. With this dual pre-embedding method, peroxidase-stained axons containing the inhibitory neurotransmitter γ-aminobutyric acid were found to synapse directly on silver-stained dopamine neurons in the rat dorsomedial hypothalamus. This approach can be used in combination with a post-embedding immunocytochemical colloidal gold procedure, allowing ultrastructural identification of three neurotransmitter-related antigens in the same tissue section.