Phenotype, and migration properties of three major subsets of tissue homing T cells in sheep
Phenotype, and migration properties of three major subsets of tissue homing T cells in sheep
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DOI：
10.1002/eji.1830261025
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发表时间：
1996-10-01
影响因子：
5.4
通讯作者：
Butcher, EC
中科院分区：
文献类型：
--
作者：
Mackay, CR;Andrew, DP;Butcher, EC
T细胞在迁移途径上表现出一定的偏向性：一些T细胞优先迁移到周围淋巴结(LN)，一些迁移到粘膜组织，一些迁移到周围组织，如皮肤。通过收集流入和流出外周和肠道LN的淋巴，并使用荧光染料追踪淋巴细胞的再循环，在绵羊身上检查了这些再循环途径。抗α4、β1和β7整合素的单抗和L-选择素被用来确定循环T细胞的三个主要群体。幼稚T细胞(L-选择素(+)、α4β1(Lo)β7(Lo))优先通过外周LN迁移。可以定义两个记忆群体：α4β1(Hi)β7(-)和α4β7(Hi)beta 1(Lo)。从皮肤流出的淋巴中有α4β1(Hi)β7(-)T细胞。优先通过肠LN的T细胞为α4β7(Hi)β1(Lo)。与此迁移模式相一致的是，肠LN和Peyer‘s斑内高内皮微静脉表达α4β7的内皮细胞受体MAdCAM-1，而外周型LN高内皮微静脉表达MAdCAM-1。因此，根据整合素的表达，至少有三个容易定义的T细胞亚群显示出不同的迁移偏好。
T cells show a bias in their migration pathways: some T cells preferentially migrate to peripheral lymph nodes (LN), some to mucosal tissues, and some to peripheral tissues such as skin. These recirculation pathways were examined in sheep by collecting lymph draining into and out of peripheral and intestinal LN, and using fluorescent dyes to trace the recirculation of the lymph cells. Monoclonal antibodies (mAb) to alpha 4, beta 1, and beta 7 integrins, and L-selectin, were used to define three major populations of recirculating T cells. Naive-type T cells (L-selectin(+), alpha 4 beta 1(lo)beta 7(lo)) migrated preferentially through peripheral LN. Two memory populations could be defined: alpha 4 beta 1(hi)beta 7(-) and alpha 4 beta 7(hi)beta 1(lo). alpha 4 beta 1(hi)beta 7(-) T cells were present in lymph draining from the skin. T cells migrating preferentially through intestinal LN were alpha 4 beta 7(hi)beta 1(lo). Consistent with this migration pattern, the endothelial receptor for alpha 4 beta 7, mucosal addressin cell adhesion molecule-1 (MAdCAM-1) was detected on high endothelial venules within intestinal LN and Peyer's patches, but only weakly on high endothelial venules within peripheral LN. Thus, there are at least three easily definable subsets of T cells, based on integrin expression, which show distinct migration preferences.