HYBRID CELLS DERIVED FROM FUSION OF TA3-HA ASCITES-CARCINOMA WITH NORMAL FIBROBLASTS .1. MALIGNANCY, KARYOTYPE, AND FORMATION OF ISOANTIGENIC VARIANTS
HYBRID CELLS DERIVED FROM FUSION OF TA3-HA ASCITES-CARCINOMA WITH NORMAL FIBROBLASTS .1. MALIGNANCY, KARYOTYPE, AND FORMATION OF ISOANTIGENIC VARIANTS
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DOI：
10.1093/jnci/50.5.1259
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发表时间：
1973-01-01
期刊：
影响因子：
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通讯作者：
HARRIS, H
中科院分区：
文献类型：
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作者：
KLEIN, G;FRIBERG, S;HARRIS, H
将高度恶性的 TA3-Ha 小鼠腹水癌细胞与二倍体 A.CA 成纤维细胞融合产生的杂交体在遗传相容的新生 X 射线照射 (A X A.CA)F1 小鼠中通常具有较低的摄取率。这证实了之前的研究，即二倍体成纤维细胞抑制与其融合的肿瘤细胞的恶性表型。对许多克隆杂交群体进行了测试，除了一种之外，在细胞体外渐进繁殖后，所有细胞都重新出现了高度恶性的行为。恶性表型的再现常常与体外可检测到的染色体丢失同时发生，但即使对于体外未检测到染色体总数显着减少的细胞群，由于染色体已被消除的细胞选择性过度生长而产生了肿瘤。为了从杂交细胞群中选择同抗原丢失变体，将细胞注射到亲本品系小鼠中。结果显示出明显的不对称性。 H-2a 兼容变体很容易分离，但没有获得特异性 H-2f 兼容品系。据推测，携带 H-2 基因座的 TA3 衍生的第九染色体参与控制恶性行为所需的某些细胞过程。携带H-2基因座的A.CA衍生染色体显然不是恶性表型表达所必需的。
Hybrids produced by fusing cells of the highly malignant TA3-Ha mouse ascites carcinoma with diploid A.CA fibroblasts generally gave low-take incidences in genetically compatible newborn X-irradiated (A X A.CA)F1mice. This confirmed previous work in which diploid fibroblasts suppressed the malignant phenotype of tumor cells with which they were fused. A number of clonal hybrid populations were tested and, in all except one, highly malignant behavior reappeared after progressive propagation of the cells in vitro. The reappearance of the malignant phenotype was frequently paralleled by detectable chromosome losses in vitroi but even with cell populations in which no significant decrease in total chromosome number was detected in vitro, tumors were produced by the selective overgrowth of cells from which chromosomes had been eliminated. To select isoantigenic loss variants from the hybrid cell populations, the cells were injected into the parental strain mice. The resultsshowed marked asymmetry. H-2a-compatible variants were isolated readily, but no specifically H-2f-compatible lines were obtained. It was hypothesized that the TA3-derived ninth chromosome carrying theH-2alocus is involved in control of some cellular process required for malignant behavior. The A.CA-derived chromosome carrying theH-2flocus is obviously not required for expression of the malignant phenotype.