Biosynthesis of Quinolidomicin, the Largest Known Macrolide of Terrestrial Origin: Identification and Heterologous Expression of a Biosynthetic Gene Cluster over 200 kb
Biosynthesis of Quinolidomicin, the Largest Known Macrolide of Terrestrial Origin: Identification and Heterologous Expression of a Biosynthetic Gene Cluster over 200 kb
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DOI：
10.1021/acs.orglett.8b03570
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发表时间：
2018-12-21
期刊：
影响因子：
5.2
通讯作者：
Shin-ya, Kazuo
中科院分区：
文献类型：
--
作者：
Hashimoto, Takuya;Hashimoto, Junko;Shin-ya, Kazuo
喹诺霉素A（1）是陆地来源的最大的大环内酯类化合物，由60元环组成，其生物合成基因簇超过200 kb。克隆并异源表达了喹诺酮类抗生素的基因簇。产物的确认导致结构修改，其中报告结构的发色团部分中的羟基被胺基取代。
Quinolidomicin A(1) is the largest macrolide compound from terrestrial sources, consisting of a 60-membered ring, and its biosynthetic gene cluster was revealed to be over 200 kb. The gene cluster for quinolidomicin was cloned and heterologously expressed. Confirmation of the product led to a structural revision, in which the hydroxy group in the chromophore moiety of the reported structure was replaced by an amine group.