The mitochondrial common deletion in Parkinson's disease and related movement disorders.
The mitochondrial common deletion in Parkinson's disease and related movement disorders.
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帕金森病和相关运动障碍中线粒体常见缺失。
DOI：
10.1016/s1353-8020(01)00041-4
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发表时间：
2002
影响因子：
4.1
通讯作者：
Perry,G
中科院分区：
文献类型：
--
作者：
Zhang,J;Montine,TJ;Smith,MA;Siedlak,SL;Gu,G;Robertson,D;Perry,G
线粒体4977-bp共同缺失已在一些研究中报道，专门发生或与帕金森病（PD）患者的中脑频率增加。其他研究无法证实这些结果;相反，有人认为线粒体常见缺失与中脑的衰老有关，而不是PD。这些相互矛盾的结果的一个可能的解释是难以量化整个中脑或黑质（SN）中的线粒体DNA缺失或突变，而只有一个子集的中脑神经元在PD中退化。此外，没有一项研究涉及中脑内常见缺失的细胞类型。在这项研究中，我们使用原位杂交技术检测常见的缺失，在部分中脑PD患者，多系统萎缩-帕金森病型（MSA-P），进行性核上性麻痹（PSP），路易体痴呆（DLB），年龄匹配的控制，和个人的不同年龄。结果表明，在SN和其他中脑区域中，线粒体共同缺失主要在神经元中积累，而不是在胶质细胞中积累。PD、MSA-P、PSP、DLB患者或年龄匹配的对照组中，黑质神经元中常见缺失的神经元数量或分布或常见缺失的平均染色密度（AMD）无显着差异。此外，在任何年龄组之间，黑质神经元中常见缺失的神经元的数量或分布没有差异，尽管在老年患者中非黑质神经元中常见缺失有增加的趋势。这些数据表明，4977 bp的共同缺失在中脑线粒体DNA中的积累主要发生在神经元中，并且通过这种细胞学方法，它与常见的运动障碍或衰老中的黑质神经变性无关。
The mitochondrial 4977-bp common deletion has been reported in some studies to occur exclusively or with increased frequency in the midbrain of patients with Parkinson's disease (PD). Other studies could not confirm these results; rather, it was suggested that the mitochondrial common deletion is associated with aging in the midbrain and not PD. One possible explanation for these conflicting results is the difficulty in quantifying mitochondrial DNA deletions or mutations in the whole midbrain or substantia nigra (SN) while only a subset of midbrain neurons degenerate in PD. In addition, none of the studies has addressed the cell types with the common deletion within the midbrain. In this study we used in situ hybridization to detect the common deletion in sections of midbrain from patients with PD, multiple system atrophy-parkinsonian type (MSA-P), progressive supranuclear palsy (PSP), dementia with Lewy bodies (DLB), age-matched controls, and individuals of different ages. The results demonstrated that the mitochondrial common deletion accumulated primarily in neurons but not glia in both the SN and other midbrain regions. There was no significant difference in the number or distribution of neurons with the common deletion or the average of the mean densities (AMD) of staining with the common deletion in nigral neurons among patients with PD, MSA-P, PSP, DLB, or age-matched controls. In addition, there was no difference in the number or distribution of neurons with the common deletion in nigral neurons between any age group, although there was a tendency for the common deletion to increase in the non-nigral neurons in older patients. These data indicate that accumulation of the 4977-bp common deletion in mitochondrial DNA in midbrain occurred primarily in neurons, and by this cytological approach, it was not associated with nigral neurodegeneration in the common movement disorders or aging.
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竞争性聚合酶链反应表明，帕金森病黑质中的“常见缺失”并未增加
影响因子：
8.6
作者：
S. Kösel;R. Egensperger;N. Schnopp;M. Graeber
通讯作者：
M. Graeber
影响因子：
14.9
作者：
HOLT, IJ;HARDING, AE;MORGANHUGHES, JA
通讯作者：
MORGANHUGHES, JA
影响因子：
6
作者：
Zhang, J;Perry, G;Montine, TJ
通讯作者：
Montine, TJ
帕金森病中的自由基和线粒体功能障碍。
影响因子：
3.9
作者：
A. Schapira;A. Hartley;M. Cleeter;J. Cooper
通讯作者：
J. Cooper
血小板的电子转移复合物 I 和 IV 在帕金森病中异常，但在帕金森综合征中正常。
DOI：
--
发表时间：
1993
期刊：
Brain : a journal of neurology
影响因子：
--
作者：
Reiner Benecke;P. Strümper;Hanns Weiss
通讯作者：
Hanns Weiss