INHIBITORY NEURONS OF A MOTOR PATTERN GENERATOR IN XENOPUS REVEALED BY ANTIBODIES TO GLYCINE
INHIBITORY NEURONS OF A MOTOR PATTERN GENERATOR IN XENOPUS REVEALED BY ANTIBODIES TO GLYCINE
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DOI：
10.1038/324255a0
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发表时间：
1986-11-20
期刊：
影响因子：
64.8
通讯作者：
STORMMATHISEN, J
中科院分区：
文献类型：
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作者：
DALE, N;OTTERSEN, OP;STORMMATHISEN, J
甘氨酸和γ-氨基丁酸（GABA）是重要的抑制性递质1，2。而神经元使用GABA作为递质可以可视化的免疫细胞化学方法谷氨酸脱羧酶（GAD）3，4或GABA 5 -8，没有可比的技术已可用于选择性可视化的甘氨酸能神经元。我们现在已经产生了多克隆抗体，它特异性地识别戊二醛固定组织中的甘氨酸。我们使用这些抗体来研究甘氨酸在theXenopus胚胎的简单中枢神经系统（CNS）中的分布，其中包含解剖学和生理学上定义的一类相互抑制的中间神经元，连合中间神经元9 -11。这些中间神经元在游泳运动模式的产生中具有重要作用，并且被认为是甘氨酸能的11 -13。甘氨酸抗体特异性染色这些神经元间，揭示其在胚胎中枢神经系统中的分布和数量。这是第一次证明甘氨酸样免疫反应性的选择性定位在一个假定的甘氨酸能类神经元，其特征在于生理学，神经病学和解剖学。
Glycine and γ-aminobutyric acid (GABA) are inhibitory transmitters of major importance1,2. Whereas neurones using GABA as the transmitter can be visualized by immunocytochemical methods for glutamate decarboxylase (GAD)3,4or GABA5–8, no comparable techniques have been available for the selective visualization of glycinergic neurones. We have now produced polyclonal antibodies which specifically recognize glycine in glutaral-dehyde-fixed tissue. We used these antibodies to investigate the distribution of glycine in the simple central nervous system (CNS) of theXenopusembryo, which contains an anatomically and physiologically defined class of reciprocal inhibitory interneurones, the commissural interneurones9–11. These interneurones have an important role in the generation of the swimming motor pattern and are thought to be glycinergic11–13. The glycine antibodies specifically stain these interneurones, revealing their distribution and number in the embryo CNS. This is the first demonstration of the selective localization of glycine-like immunoreactivity in a putative glycinergic class of neurone that has been characterized physiologically, pharmacologically and anatomically.