Tyrosine kinase activity of nerve growth factor and estrogen in embryonic septal neurons cultured from the rat.
Tyrosine kinase activity of nerve growth factor and estrogen in embryonic septal neurons cultured from the rat.
复制标题
大鼠胚胎间隔神经元中神经生长因子和雌激素的酪氨酸激酶活性。
DOI：
10.1023/a:1021651530846
复制
发表时间：
2002
影响因子：
4.4
通讯作者：
Mudd,LauraM
中科院分区：
文献类型：
--
作者：
Green,Dallis;Jonusas,Alex;Montague,JeremyR;Mudd,LauraM
Alzheimer's disease (AD) is a neurodegenerative disease characterized by dementia, senile plaques, fibrillary tangles, and a reduction of cholinergic neurons in the septal nucleus of the brain. Nerve growth factor (NGF) and estrogen were studied to observe effects on tyrosine kinase activity in septal neurons. The time course of tyrosine kinase activation and number of cells in which tyrosine kinase was activated were measured. Tissue from embryonic day 16 rats was microdissected and the septal neurons obtained were treated with estrogen (10 μM) or NGF (100 ng/mL) at intervals of 1, 2, 3, 4, 5, or 10 min. Immunostaining for phosphotyrosine revealed that cells treated with NGF showed an increase in phosphotyrosine activity within 2-4 min followed by a decline to control levels of enzyme activity. Treatment with estrogen led to an increase in phosphotyrosine immunostaining within 2-3 min followed by a decline to control levels. This time course suggests a mechanism for estrogen activity other than the traditional method involving binding to nuclear receptors followed by protein synthesis.
