Retrograde regulation of nuclear gene expression in CW-CMS of rice
Retrograde regulation of nuclear gene expression in CW-CMS of rice
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DOI：
10.1007/s11103-006-9097-8
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发表时间：
2007-02-01
影响因子：
5.1
通讯作者：
Toriyama, Kinya
中科院分区：
文献类型：
--
作者：
Fujii, Sota;Komatsu, Setsuko;Toriyama, Kinya
cm -细胞质雄性不育系具有Oryza rufipogon Griff的细胞质，成熟花粉在光学显微镜下形态正常，但缺乏萌发能力；恢复基因Rf17被确定为恢复这种能力的基因。比较了CW-CMS系[cms-CW] rf17rf17与正常细胞质保持系[normal] rf17rf17成熟花药核基因表达的差异。利用22 k的水稻寡核苷酸阵列，我们检测到58个基因在CW-CMS系中上调了3倍以上。利用RT-PCR进一步研究了20个基因在其他器官中的表达。在[cm - cw] rf17rf17和[cm - cw] rf17rf17中，包括选择性氧化酶基因在内的5个基因优先表达，而在[normal] rf17rf17和[cm - cw] rf17rf17中不优先表达。这种[cms-CW] rf17rf17特异性基因表达仅在成熟花药中观察到，而在叶、茎和根中均未观察到，说明存在花药特异性线粒体逆行调控核基因表达，Rf17具有恢复异位基因表达的作用。我们还使用蛋白质组学方法来发现逆行调节蛋白，并鉴定了6种不同积累的蛋白。这些结果揭示了影响核基因表达的器官特异性诱导线粒体逆行通路可能与CMS有关。
The CW-cytoplasmic male sterility (CMS) line has the cytoplasm of Oryza rufipogon Griff, and mature pollen is morphologically normal under an optical microscope but lacks the ability to germinate; restorer gene Rf17 has been identified as restoring this ability. The difference between nuclear gene expression in mature anthers was compared for the CW-CMS line, [cms-CW] rf17rf17, and a maintainer line with normal cytoplasm of Oryza sativa L., [normal] rf17rf17. Using a 22-k rice oligoarray we detected 58 genes that were up-regulated more than threefold in the CW-CMS line. Expression in other organs was further investigated for 20 genes using RT-PCR. Five genes, including genes for alternative oxidase, were found to be preferentially expressed in [cms-CW] rf17rf17 but not in [normal] rf17rf17 or [cms-CW] Rf17Rf17. Such [cms-CW] rf17rf17-specific gene expression was only observed in mature anthers but not in leaves, stems, or roots, indicating the presence of anther-specific mitochondrial retrograde regulation of nuclear gene expression, and that Rf17 has a role in restoring the ectopic gene expression. We also used a proteomic approach to discover the retrograde regulated proteins and identified six proteins that were accumulated differently. These results reveal organ-specific induced mitochondrial retrograde pathways affecting nuclear gene expression possibly related to CMS.