STRUCTURE OF NATIVE RENILLA-RENIFORMIS LUCIFERIN
STRUCTURE OF NATIVE RENILLA-RENIFORMIS LUCIFERIN
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DOI：
10.1073/pnas.74.10.4285
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发表时间：
1977-01-01
影响因子：
11.1
通讯作者：
CORMIER, MJ
中科院分区：
文献类型：
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作者：
HORI, K;CHARBONNEAU, H;CORMIER, MJ
通过对合成的荧光素和直接参与生物发光系统的蛋白质的质谱分析，确定了来自生物发光腔肠动物的天然荧光素的结构为3，7-dihydro-2-(p-hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazo[1，2-a]pyrazin-3-one。先前关于荧光素分子量的报道是不正确的，如重新检查光谱数据和合成2个衍生物所显示的那样。对分离和合成的荧光素的动力学、发射和量子产率数据的详细分析证实了这种结构。在来自不同门的许多物种中证实了这种结构，这表明各种生物发光海洋生物都有共同的底物。
The structure of native luciferin from the bioluminescent coelenterate R. reniformis is 3,7-dihydro-2-(p-hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazo[1,2-a]pyrazin-3-one by mass spectral analysis of synthetic luciferin and the luciferin derived from a protein directly involved in the bioluminescent system. A previous report of the MW of luciferin is incorrect as shown by reexamination of the spectral data and by synthesis of 2 derivatives. Detailed analysis of kinetic, emission and quantum yield data for the isolated and synthetic luciferins confirms this structure. Confirmation of this structure in a number of species from different phyla suggests a common substrate for a variety of bioluminescent marine organisms.