Dynamics of fecal microbial communities in children with diarrhea of unknown etiology and genomic analysis of associated Streptococcus lutetiensis.
Dynamics of fecal microbial communities in children with diarrhea of unknown etiology and genomic analysis of associated Streptococcus lutetiensis.
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DOI：
10.1186/1471-2180-13-141
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发表时间：
2013-06-19
期刊：
影响因子：
4.2
通讯作者：
Xu J
中科院分区：
文献类型：
--
作者：
Jin D;Chen C;Li L;Lu S;Li Z;Zhou Z;Jing H;Xu Y;Du P;Wang H;Xiong Y;Zheng H;Bai X;Sun H;Wang L;Ye C;Gottschalk M;Xu J
从粪便样品中提取的16S rRNA基因序列提供了对粪便微生物群落动态的见解。这可能为那些病情被归因于未知病原体的患者提供有价值的病因学信息，而使用常规培养方法无法实现这一点。我们研究了2006年山西省儿童医院收治的33例腹泻患儿。33例腹泻患儿中有19例不能用常规培养和聚合酶链反应方法进行病原学诊断。19例不明原因腹泻患儿中有11例在入院时以链球菌为最主要的粪便细菌属。在连续采集三份粪便样本的9名儿童中，有8名儿童的链球菌是主要的粪便细菌属，包括3名牛链球菌组和3名链球菌属，在恢复期间和恢复后都有所减少。我们分离了可能来自S.牛链球菌组，然后鉴定为来自一名儿童的藤黄链球菌和来自一名儿童的解没食子酸链球菌亚种。两个孩子的巴氏杆菌我们对S. lutetiensis，并确定了五个抗生素岛，两个致病性岛和五个独特的基因组岛。鉴定的毒力基因包括无乳链球菌溶血毒素cylZ和与致病性链球菌定殖相关的分选酶。我们确定了S。lutetiensis和S.解没食子酸亚种从不明原因腹泻患儿中分离巴氏杆菌，并在其基因组中发现致病岛和毒力基因。卢特人
The sequences of the 16S rRNA genes extracted from fecal samples provide insights into the dynamics of fecal microflora. This potentially gives valuable etiological information for patients whose conditions have been ascribed to unknown pathogens, which cannot be accomplished using routine culture methods. We studied 33 children with diarrhea who were admitted to the Children’s Hospital in Shanxi Province during 2006. Nineteen of 33 children with diarrhea could not be etiologically diagnosed by routine culture and polymerase chain reaction methods. Eleven of 19 children with diarrhea of unknown etiology had Streptococcus as the most dominant fecal bacterial genus at admission. Eight of nine children whom three consecutive fecal samples were collected had Streptococcus as the dominant fecal bacterial genus, including three in the Streptococcus bovis group and three Streptococcus sp., which was reduced during and after recovery. We isolated strains that were possibly from the S. bovis group from feces sampled at admission, which were then identified as Streptococcus lutetiensis from one child and Streptococcus gallolyticus subsp. pasteurianus from two children. We sequenced the genome of S. lutetiensis and identified five antibiotic islands, two pathogenicity islands, and five unique genomic islands. The identified virulence genes included hemolytic toxin cylZ of Streptococcus agalactiae and sortase associated with colonization of pathogenic streptococci. We identified S. lutetiensis and S. gallolyticus subsp. pasteurianus from children with diarrhea of unknown etiology, and found pathogenic islands and virulence genes in the genome of S. lutetiensis.
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影响因子：
3.6
作者：
Gerber, Jeffrey S.;Glas, Mary;Shah, Samir S.
通讯作者：
Shah, Samir S.
DOI：
10.1053/j.spid.2005.12.008
发表时间：
2005-04-01
期刊：
Seminars in pediatric infectious diseases
影响因子：
--
作者：
O'Ryan, Miguel;Prado, Valeria;Pickering, Larry K
通讯作者：
Pickering, Larry K
影响因子：
3.1
作者：
Spellerberg, B;Rozdzinski, E;Podbielski, A
通讯作者：
Podbielski, A
影响因子：
3.6
作者：
Holmes, AR;McNab, R;Jenkinson, HF
通讯作者：
Jenkinson, HF
影响因子：
4.4
作者：
O'Toole, Paul W.;Snelling, William J.;Bentley, Stephen D.
通讯作者：
Bentley, Stephen D.