Mapping of HIV-1C Transmission Networks Reveals Extensive Spread of Viral Lineages Across Villages in Botswana Treatment-as-Prevention Trial
Mapping of HIV-1C Transmission Networks Reveals Extensive Spread of Viral Lineages Across Villages in Botswana Treatment-as-Prevention Trial
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DOI：
10.1093/infdis/jiaa276
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发表时间：
2020-11-15
影响因子：
6.4
通讯作者：
Essex, M.
中科院分区：
文献类型：
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作者：
Novitsky, Vlad;Zahralban-Steele, Melissa;Essex, M.
背景系统发育图谱的HIV-1谱系传播的定义的地理位置是有希望的，更好地了解艾滋病毒的传播网络，以设计最佳的预防干预措施。我们从艾滋病毒感染者（PLWH）中获得了接近全长的HIV-1基因组序列，包括2013-2018年在博茨瓦纳30个社区进行的博茨瓦纳联合预防项目中接受抗逆转录病毒治疗的参与者。用最大似然法估计病毒序列间的系统发育关系。我们从6075例PLWH中获得了6078个接近全长的HIV-1C基因组序列。截至2018年年中，我们确定了在博茨瓦纳传播的984个遗传学上不同的HIV-1谱系（分子HIV簇），每个簇有2-27个成员。其中，二分体占62%，约32%（n = 316）被发现在单一社区，68%（n = 668）分布在多个社区。集群中的男性比女性大3岁左右（中位年龄42岁对39岁; P
Background. Phylogenetic mapping of HIV-1 lineages circulating across defined geographical locations is promising for better understanding HIV transmission networks to design optimal prevention interventions.Methods. We obtained near full-length HIV-1 genome sequences from people living with HIV (PLWH), including participants on antiretroviral treatment in the Botswana Combination Prevention Project, conducted in 30 Botswana communities in 2013-2018. Phylogenetic relationships among viral sequences were estimated by maximum likelihood.Results. We obtained 6078 near full-length HIV-1C genome sequences from 6075 PLWH. We identified 984 phylogenetically distinct HIV-1 lineages (molecular HIV clusters) circulating in Botswana by mid-2018, with 2-27 members per cluster. Of these, dyads accounted for 62%, approximately 32% (n = 316) were found in single communities, and 68% (n = 668) were spread across multiple communities. Men in clusters were approximately 3 years older than women (median age 42 years, vs 39 years; P