Proteomic analysis of differentially expressed proteins of Nicotiana benthamiana triggered by INF1 elicitin from Phytophthora infestans
Proteomic analysis of differentially expressed proteins of Nicotiana benthamiana triggered by INF1 elicitin from Phytophthora infestans
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致病疫霉 INF1 诱导素触发的本塞姆氏烟草差异表达蛋白的蛋白质组学分析
DOI：
10.1007/s10327-017-0699-6
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发表时间：
2017-03-01
影响因子：
1.2
通讯作者：
Liu, Jun
中科院分区：
文献类型：
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作者：
Du, Juan;Guo, Xiao;Liu, Jun
病原体相关分子模式(PAMP)触发的免疫(PTI)被认为是持久的，因为PAMP在整个微生物类别中都是保守的。ELICTINS是卵菌属中结构保守的胞外蛋白，具有PAMPs的特征。INF1是晚疫病病原菌马铃薯晚疫病菌分泌的一种elicitin蛋白。最近在马铃薯中克隆了一个介导INF1反应的细胞表面受体样蛋白。此外，据报道，其他一些基因也参与了INF1触发的免疫反应；然而，INF1触发免疫的分子机制仍然知之甚少。在这里，我们使用基于相对和绝对量化的等压标记定量蛋白质组学来分析参与INF1触发的烟草细胞死亡反应的蛋白质。我们的方法鉴定了2964种蛋白质，其中32种在INF1诱导后丰度发生了显著变化。病毒诱导的基因沉默分析表明，八种上调蛋白中的两种，即ATP依赖转运蛋白和60S核糖体蛋白L15，在INF1诱导的细胞死亡反应中是必不可少的。
Pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) is considered to be durable, given that PAMPs are conserved in entire classes of microbes. Elicitins are structurally conserved extracellular proteins in oomycete species and are well characterized as having features of PAMPs. INF1 is an elicitin protein secreted by the late blight pathogen Phytophthora infestans. A cell surface receptor-like protein that mediates INF1 response was recently cloned in potato. In addition, some other genes are reportedly involved in INF1-triggered immune responses; however, the molecular mechanisms of INF1-triggered immunity remain poorly understood. Here, we used isobaric tags for relative and absolute quantification-based quantitative proteomics to analyze proteins involved in INF1-triggered cell death responses in Nicotiana benthamiana. Our approach identified 2964 proteins, 32 of which were significantly altered in abundance after INF1 induction. Two of eight selected upregulated proteins, namely, ATP dependent transporter and 60S ribosomal protein L15 were shown to be essential in INF1-triggered cell death responses by virus-induced gene silencing analysis.