Detection of rotaviruses in the day care environment by reverse transcriptase polymerase chain reaction.
Detection of rotaviruses in the day care environment by reverse transcriptase polymerase chain reaction.
复制标题
通过逆转录酶聚合酶链反应检测日托环境中的轮状病毒。
DOI：
10.1093/infdis/166.3.507
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Yolken,R
中科院分区：
文献类型：
--
作者：
Wilde,J;Van,R;Pickering,L;Eiden,J;Yolken,R
A组轮状病毒是日托中心婴幼儿发病的重要原因。粪-口途径传播是公认的，但污染物和环境表面也可能在传播中发挥重要作用。一种高灵敏度的聚合酶链反应（Pf.R）检测轮状病毒RNA在日托环境。采样区域包括地板、换尿布区域、玩具球和其他表面。在两个发生轮状病毒爆发的中心，18个玩具球中有7个（39%）检测到轮状病毒，39个环境表面拭子中有8个（21%）检测到轮状病毒。相比之下，在没有轮状病毒爆发的中心，21个玩具球中只有1个（5%）和44个环境表面拭子中只有1个（2%）可检测到轮状病毒（P =.0001）。因此，轮状病毒在腹泻爆发期间在日托中心高度流行。通过灵敏的核酸扩增技术监测环境可能导致减少日托环境中疾病传播的策略。
Group A rotavirus is an important cause of morbidity among infants and toddlers in day care centers. Transmission by the fecal-oral route is well established, but fomites and environmental surfaces may also play an important role in transmission. A highly sensitive polymerase chain reaction (Pf.R) assay was used to detect rotavirus RNA in day care environments. Areas sampled included floors, diaper change areas, toy balls, and other surfaces. In two centers undergoing outbreaks of rotavirus, 7 (39%)of 18 toy balls had detectable rotavirus as did 8 (21%)of 39 swabs from environmental surfaces. By comparison, only 1 (5%) of 21 toy balls and 1 (2%) of 44 environmental surface swabs had detectable rotavirus in centers without rotavirus outbreaks (P =.0001). Thus, rotaviruses are highly prevalent in day care centers during outbreaks of diarrhea. The monitoring of environments by sensitive nucleic acid amplification techniques may lead to strategies for the diminution of disease transmission within the day care environment.