The human homolog of the JE gene encodes a monocyte secretory protein.
The human homolog of the JE gene encodes a monocyte secretory protein.
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JE 基因的人类同源物编码单核细胞分泌蛋白。
DOI：
10.1128/mcb.9.11.4687-4695.1989
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发表时间：
1989
影响因子：
5.3
通讯作者：
Wong,GG
中科院分区：
文献类型：
--
作者：
Rollins,BJ;Stier,P;Ernst,T;Wong,GG
小鼠成纤维细胞基因 JE 是最早被描述的血小板衍生生长因子诱导基因之一。 JE（mJE）编码的蛋白质是一个分泌型细胞因子样糖蛋白大家族的原型，其所有成员均由单核细胞、巨噬细胞和T淋巴细胞中的有丝分裂或激活信号诱导；JE是在成纤维细胞中唯一被鉴定的成员。我们报告了从人成纤维细胞中克隆的鼠乙型脑炎的人类同源物的鉴定。由人JE（hJE）编码序列预测的蛋白质比mJE短55个氨基酸，其序列与最近纯化的单核细胞趋化剂的序列相同。当在COS细胞中表达时，人JE cDNA指导分泌Mr16,000至18,000的N-糖基化蛋白以及Mr15,500、15,000和13,000的蛋白。针对 mJE 的抗体可识别这些 hJE 物种，所有这些 hJE 物种均由人类成纤维细胞分泌。在佛波醇肉豆蔻酸酯乙酸酯诱导的单核细胞分化过程中，HL60 细胞中的 hJE 表达受到刺激。然而，静息的人单核细胞持续分泌 hJE；用γ干扰素处理不会增强单核细胞中的hJE表达，而用佛波醇肉豆蔻酸酯乙酸酯或脂多糖处理可抑制其表达。因此，人类JE编码JE相关细胞因子样蛋白大家族的另一个成员，在本例中是一种新型的人类单核细胞和成纤维细胞分泌蛋白。
The mouse fibroblast gene, JE, was one of the first platelet-derived growth factor-inducible genes to be described as such. The protein encoded byJE(mJE) is the prototype of a large family of secreted, cytokinelike glycoproteins, all of whose members are induced by a mitogenic or activation signal in monocytes, macrophages, and T lymphocytes;JEis the only member to have been identified in fibroblasts. We report the identification of a human homolog for murine JE, cloned from human fibroblasts. The protein predicted by the coding sequence of human JE (hJE) is 55 amino acids shorter than mJE, and its sequence is identical to that of a recently purified monocyte chemoattractant. When expressed in COS cells, the human JE cDNA directed the secretion of N-glycosylated proteins ofMr16,000 to 18,000 as well as proteins ofMr15,500, 15,000, and 13,000. Antibodies raised against mJE recognized these hJE species, all of which were secreted by human fibroblasts. hJE expression was stimulated in HL60 cells during phorbol myristate acetate-induced monocytoid differentiation. However, resting human monocytes constitutively secreted hJE; treatment with gamma interferon did not enhance hJE expression in monocytes, and treatment with phorbol myristate acetate or lipopolysaccharide inhibited its expression. Thus, humanJEencodes yet another member of the large family of JE-related cytokinelike proteins, in this case a novel human monocyte and fibroblast secretory protein.