Polyclonal Antibody Against Egg Yolk Extracts of Medaka, Oryzias latipes (Teleostei), for Investigating the Influences of Xeno-Estrogens
Polyclonal Antibody Against Egg Yolk Extracts of Medaka, Oryzias latipes (Teleostei), for Investigating the Influences of Xeno-Estrogens
复制标题
针对青鳉、Oryzias latipes (Teleostei) 蛋黄提取物的多克隆抗体，用于研究异种雌激素的影响
DOI：
10.11403/jset.3.15
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Y. Magara
中科院分区：
文献类型：
--
作者：
A. Tabata;S. Kashiwada;Nobukazu Miyamot;H. Ishikawa;Y. Ohnishi;M. lTOH;Y. Magara
获得了兔抗龙舌兰蛋黄提取物的血清。从E -暴露雄性medaka的腹水中纯化medaka的卵黄原蛋白（Vitellogenin， VTG）。采用双扩散分析和免疫点分析对其免疫特性进行了检验。所获得的抗血清对暴露的medaka（雄性）的血液和腹水、纯化的medaka VTG以及雌性medaka的蛋黄提取物、血液和肝细胞提取物均有免疫反应，但对雄性medaka的血液和肝细胞提取物或鲤鱼VTG均无免疫反应。在双扩散分析中，腹水中有两种蛋白与抗血清发生反应。在原生PAGE上还观察到除VTG外的一些蛋白。结果表明，E诱导的蛋白不仅仅是VTG。所获得的抗血清对暴露于E、对壬基酚或双酚a的雄性medaka血液有反应。这些结果表明，该抗血清可用于研究外源雌激素对medaka的影响。
Rabbit antiserum against medaka eggyolk extract was obtained. Vitellogenin (VTG) of medaka was purified from ascites of E,-exposed male medaka. Immuno-character was tested by double diffusion analysis and immuno-dot analysis. The antiserum obtained exhibited an immuno-reaction with blood and ascites of E,-exposed medaka (male), purified medaka VTG, and egg yolk extracts, blood and hepatocyte extracts of female medaka, but not with blood and hepatocyte extracts of male medaka or carp VTG. In double diffusion analysis, two kinds of protein in the ascites reacted to the antiserum. A few proteins other than VTG were also observed by native PAGE. The results suggest that the protein induced by E, is not only VTG. The antiserum obtained reacted to blood of male medaka exposed to E, p-nonylphenol or bisphenol-A. Those results were suggested that the antiserum is useful for investigating the influences of xeno-estrogens on medaka.