Molecular Characterization of Reduced Susceptibility to Biocides in Clinical Isolates of Acinetobacter baumannii.
Molecular Characterization of Reduced Susceptibility to Biocides in Clinical Isolates of Acinetobacter baumannii.
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鲍曼不动杆菌临床分离株对杀菌剂敏感性降低的分子特征
DOI：
10.3389/fmicb.2017.01836
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发表时间：
2017
影响因子：
5.2
通讯作者：
Ling B
中科院分区：
文献类型：
--
作者：
Lin F;Xu Y;Chang Y;Liu C;Jia X;Ling B
主动外排被认为是抗生素和杀生物剂抗性的常见机制。然而，许多药物外排泵在鲍曼不动杆菌的杀菌剂耐药性中的作用仍然未知。利用抗杀菌剂A.在鲍曼不动杆菌临床分离株中，我们通过PCR和DNA测序研究了11种已知/推定的抗菌药物耐药外排泵基因（adeB、adeG、adeJ、adeT 1、adeT 2、amvA、abeD、abeM、qacE、qacEΔ1和aceI）和三氯生靶基因fabI的发生率。逆转录酶定量PCR进行评估外排泵基因表达和三氯生或洗必泰的敏感性降低之间的相关性。助理鲍曼不动杆菌分离株显示出对三氯生、氯己定、苯扎氯铵、过氧化氢和乙醇的高度降低的敏感性。大多数测试的分离株对多种抗生素耐药。外排抗性基因在A.鲍曼不动杆菌。尽管外排泵基因表达与抗生素耐药性或杀生物剂敏感性降低之间没有明确的关系，但三氯生非敏感分离株的adeB和adeJ表达相对增加，而氯己定非敏感分离株的abeM和fabI基因表达增加。在多种抗生素耐药分离株中也证明了adeJ和abeM的表达增加。暴露的菌株亚抑制浓度的三氯生或氯己定诱导的基因表达的adeB，adeG，adeJ和fabI，和adeB，分别。FabI，Gly 95 Ser，点突变，观察到只有一个三氯生耐药菌株。在高水平三氯生耐药分离株中鉴定出具有主要克隆复合体CC 92的多种序列类型。总体而言，这项研究表明，抗生素和杀生物剂耐药性的高流行率以及复杂的交织耐药机制在临床分离的A。鲍曼不动杆菌，强调了临床抗菌药物管理和耐药性监测的重要性。
Active efflux is regarded as a common mechanism for antibiotic and biocide resistance. However, the role of many drug efflux pumps in biocide resistance in Acinetobacter baumannii remains unknown. Using biocide-resistant A. baumannii clinical isolates, we investigated the incidence of 11 known/putative antimicrobial resistance efflux pump genes (adeB, adeG, adeJ, adeT1, adeT2, amvA, abeD, abeM, qacE, qacEΔ1, and aceI) and triclosan target gene fabI through PCR and DNA sequencing. Reverse transcriptase quantitative PCR was conducted to assess the correlation between the efflux pump gene expression and the reduced susceptibility to triclosan or chlorhexidine. The A. baumannii isolates displayed high levels of reduced susceptibility to triclosan, chlorhexidine, benzalkonium, hydrogen peroxide, and ethanol. Most tested isolates were resistant to multiple antibiotics. Efflux resistance genes were widely distributed and generally expressed in A. baumannii. Although no clear relation was established between efflux pump gene expression and antibiotic resistance or reduced biocide susceptibility, triclosan non-susceptible isolates displayed relatively increased expression of adeB and adeJ whereas chlorhexidine non-susceptible isolates had increased abeM and fabI gene expression. Increased expression of adeJ and abeM was also demonstrated in multiple antibiotic resistant isolates. Exposure of isolates to subinhibitory concentrations of triclosan or chlorhexidine induced gene expression of adeB, adeG, adeJ and fabI, and adeB, respectively. A point mutation in FabI, Gly95Ser, was observed in only one triclosan-resistant isolate. Multiple sequence types with the major clone complex, CC92, were identified in high level triclosan-resistant isolates. Overall, this study showed the high prevalence of antibiotic and biocide resistance as well as the complexity of intertwined resistance mechanisms in clinical isolates of A. baumannii, which highlights the importance of antimicrobial stewardship and resistance surveillance in clinics.
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影响因子：
4.9
作者：
Fernando, Dinesh M.;Xu, Wayne;Kumar, Ayush
通讯作者：
Kumar, Ayush
DOI：
10.1073/pnas.1317052110
发表时间：
2013-12-10
影响因子：
11.1
作者：
Hassan, Karl A.;Jackson, Scott M.;Paulsen, Ian. T.
通讯作者：
Paulsen, Ian. T.
中国某教学医院耐碳青霉烯类鲍曼不动杆菌的特征
影响因子：
5.2
作者：
Chang Y;Luan G;Xu Y;Wang Y;Shen M;Zhang C;Zheng W;Huang J;Yang J;Jia X;Ling B
通讯作者：
Ling B
影响因子：
6.4
作者：
Hassan KA;Liu Q;Henderson PJ;Paulsen IT
通讯作者：
Paulsen IT
影响因子：
4.9
作者：
Curiao, Tania;Marchi, Emmanuela;Coquea, Teresa M.
通讯作者：
Coquea, Teresa M.