Effective Antisense Gene Regulation via Noncationic, Polyethylene Glycol Brushes
Effective Antisense Gene Regulation via Noncationic, Polyethylene Glycol Brushes
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DOI：
10.1021/jacs.6b05787
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发表时间：
2016-07-27
影响因子：
15
通讯作者：
Zhang, Ke
中科院分区：
文献类型：
--
作者：
Lu, Xueguang;Jia, Fei;Zhang, Ke
带负电荷的核酸通常在递送之前与聚阳离子转染剂复合。在此，我们证明了非阳离子的，生物相容性的聚合物，聚乙二醇，可以用作转染载体，通过形成刷状聚合物-DNA缀合物。刷状结构提供了具有增强的核酸酶稳定性和改善的细胞摄取的嵌入的DNA链。由于聚合物组分的生物学良性性质，未观察到细胞毒性。这种方法有可能解决寡核苷酸治疗中的几个长期挑战。
Negatively charged nucleic acids are often complexed with polycationic transfection agents before delivery. Herein, we demonstrate that a noncationic, biocompatible polymer, polyethylene glycol, can be used as a transfection vector by forming a brush polymer-DNA conjugate. The brush architecture provides embedded DNA strands with enhanced nuclease stability and improved cell uptake. Because of the biologically benign nature of the polymer component, no cytotoxicity was observed. This approach has the potential to address several long-lasting challenges in oligonucleotide therapeutics.