Isolation and characterization of a fourth Arabidopsis thaliana G-box-binding factor, which has similarities to Fos oncoprotein.
Isolation and characterization of a fourth Arabidopsis thaliana G-box-binding factor, which has similarities to Fos oncoprotein.
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DOI：
10.1073/pnas.91.7.2522
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发表时间：
1994-03
影响因子：
11.1
通讯作者：
A. Menkens;A. Cashmore
中科院分区：
文献类型：
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作者：
A. Menkens;A. Cashmore
第四个成员的拟南芥G盒结合因子（GBF）家族的bZIP蛋白，GBF4，已被分离和表征。与哺乳动物系统中的Fos相关癌蛋白类似，GBF4不能作为同源二聚体与DNA结合，尽管它含有一个能够特异性识别G盒和G盒样元件的碱性区域。然而，GBF4可以与GBF2和GBF3相互作用以异二聚体的形式结合DNA。GBF 4的亮氨酸拉链的突变表明，单个氨基酸的突变赋予蛋白质将G盒识别为同二聚体的能力，显然是通过改变亮氨酸拉链内的电荷分布。
A fourth member of the Arabidopsis G-box-binding factor (GBF) family of bZIP proteins, GBF4, has been isolated and characterized. In a manner reminiscent of the Fos-related oncoproteins of mammalian systems, GBF4 cannot bind to DNA as a homodimer, although it contains a basic region capable of specifically recognizing the G-box and G-box-like elements. However, GBF4 can interact with GBF2 and GBF3 to bind DNA as heterodimers. Mutagenesis of the leucine zipper of GBF4 indicates that the mutation of a single amino acid confers upon the protein the ability to recognize the G-box as a homodimer, apparently by altering the charge distribution within the leucine zipper.