Natural polyclonality of spontaneous AKR leukemia and its consequences for so-called specific immunotherapy.
Natural polyclonality of spontaneous AKR leukemia and its consequences for so-called specific immunotherapy.
复制标题
自发性 AKR 白血病的天然多克隆性及其对所谓特异性免疫治疗的影响。
DOI：
10.1093/jnci/62.3.623
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
P. Ebbesen
中科院分区：
文献类型：
--
作者：
L. Olsson;P. Ebbesen
通过静脉内接种10（3）个自发性AKR胸腺瘤细胞而产生白血病的年轻雌性AKR小鼠通过重复注射来自相同肿瘤的辐射细胞进行治疗。注射活细胞后1天开始处理。用来自治疗或未治疗的白血病移植小鼠的淋巴瘤细胞免疫的健康小鼠的血清和淋巴细胞的细胞毒性显示，肿瘤细胞可以被细分为四种不同的抗原类型。一种类型（克隆A）占每只自发性白血病小鼠中淋巴瘤细胞的约97%，而其余3%被细分为三种其他不同的抗原类型（克隆B、C和D）。移植性白血病治疗小鼠的淋巴瘤细胞绝不是A型克隆，而是B、C或D型克隆。重复皮下注射10（7）个来自自发性AKR胸腺瘤的辐射细胞，在移植白血病细胞的小鼠中诱导15%至34%的治愈。仅用克隆A处理诱导约32%的治愈，而用克隆B、C或D处理没有有益效果。克隆A加克隆B各用10（7）个细胞治疗，治愈率为33%;克隆A加克隆B加克隆C，治愈率为45%;所有四个克隆治愈了92%的白血病移植小鼠。免疫疗法的效率可能受到待治疗肿瘤的天然克隆性的影响。
Young female AKR mice made leukemic by iv inoculation of 10(3) spontaneous AKR thymoma cells were treated with repeated injections of irradiated cells from the same tumor. Treatment began 1 day after injection of the viable cells. The cytotoxicity of sera and lymphoid cells from healthy mice immunized with lymphoma cells from either treated or nontreated mice with leukemia grafts revealed that the tumor cells could be subdivided into four distinct antigenic types. One type (clone A) accounted for about 97% of the lymphoma cells in each mouse with spontaneous leukemia, whereas the remaining 3% were subdivided into three other distinct antigenic types (clones B, C, and D). Lymphoma cells from treated mice with grafted leukemia were never clone A type but either clone B, C, or D type. Repeated sc injections of 10(7) irradiated cells from spontaneous AKR thymomas induced from 15 to 34% cure in mice with grafts of leukemia cells. Treatment with only clone A induced about 32% cure, whereas treatment with clone B, C, or D had no beneficial effect. Treatment with 10(7) cells each of clone A plus clone B gave 33% cure; clone A plus clone B plus clone C, 45%; and all four clones cured 92% of the mice with leukemia grafts. The efficiency of immunotherapy may be influenced by the natural clonality of the tumor to be treated.