Involvement of NGATHA-Like 1 Transcription Factor in Boron Transport under Low and High Boron Conditions
Involvement of NGATHA-Like 1 Transcription Factor in Boron Transport under Low and High Boron Conditions
复制标题
NGATHA-Like 1 转录因子参与低硼和高硼条件下的硼转运
DOI：
10.1093/pcp/pcac099
复制
发表时间：
2022
影响因子：
4.9
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Tsednee,M.;Tanaka;M.;Fh Giehl;R.;von Wirテゥn;N. and Fujiwara;T.
NGATHA-Like 1 （NGAL1）转录因子已被确定为通过aug -stop介导的硼(B)依赖性翻译失速调控的基因；然而，其在B反应中的作用尚不清楚。在这里，我们发现NGAL1在拟南芥低B和高B条件下都在维持B转运中发挥重要作用。在B胁迫下，NGAL1mRNA主要在茎中积累。携带转移DNA （T-DNA）和ds转座子插入的独立突变体在空气组织中表现出B浓度降低，当B供应有限时，产生的硅质缩短和数量减少。此外，B转运蛋白基因包括结节蛋白26样内在蛋白6的表达；1 (NIP6; 1), NIP5; 1, NIP7;低B条件下，突变体中硼酸盐出口1（BOR1）显著减少，表明在B限制条件下，硼酸盐转运基因转录物积累需要NGAL1，以促进硼的运输和分布。在高B条件下，ngal1突变体表现出生长下降和芽部B浓度升高的现象。B向土壤外排所需的B转运体bor4mrna的积累在高B条件下的NGAL1植物根系中显著减少，这表明NGAL1参与了过量B下bor4的上调。我们的研究结果表明，在低B和高B条件下，NGAL1都参与了B转运体基因的转录调控，以促进B的运输和分布。
NGATHA-Like 1 (NGAL1) transcription factor has been identified as a gene regulated through AUG-stop-mediated boron (B)-dependent translation stall; however, its function in B response remains unknown. Here, we show that NGAL1 plays an important role in the maintenance of B transport under both low- and high-B conditions inArabidopsis thaliana. NGAL1mRNA is accumulated predominantly in shoots in response to B stress. Independentngal1mutants carrying transferred DNA (T-DNA) andDs-transposon insertions exhibit reduced B concentrations in aerial tissues and produce shortened and reduced number of siliques when B supply is limited. Furthermore, the expression of B transporter genes includingnodulin 26-like intrinsic protein 6; 1(NIP6;1),NIP5;1,NIP7;1andborate exporter 1(BOR1) is significantly decreased inngal1mutants under low-B condition, suggesting that NGAL1 is required for the transcript accumulation of B transporter genes to facilitate B transport and distribution under B limitation. Under high-B condition,ngal1mutants exhibit reduced growth and increased B concentration in their shoots. The accumulation ofBOR4mRNA, a B transporter required for B efflux to soil, is significantly reduced in roots ofngal1plants under high-B condition, suggesting that NGAL1 is involved in the upregulation ofBOR4in response to excess B. Together, our results indicate that NGAL1 is involved in the transcriptional regulation of B transporter genes to facilitate B transport and distribution under both low- and high-B conditions.