Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy.
Peroxiporin Expression Is an Important Factor for Cancer Cell Susceptibility to Therapeutic H2O2: Implications for Pharmacological Ascorbate Therapy.
复制标题
过氧蛋白酶表达是癌细胞对治疗性H2O2敏感性的重要因素：对药理学抗坏血酸治疗的影响。
DOI：
10.1371/journal.pone.0170442
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Rodgers VG
中科院分区：
文献类型：
--
作者：
Erudaitius D;Huang A;Kazmi S;Buettner GR;Rodgers VG
癌细胞对治疗性 H2O2 的毒性根据细胞类型的不同而有很大差异。有趣的是，已经观察到不同的癌细胞类型具有不同的过氧孔蛋白表达。我们假设过氧孔蛋白表达的变化可以改变细胞对治疗性 H2O2 浓度的敏感性。在这里，我们沉默了胰腺癌细胞系 MIA PaCa-2 上的过氧孔蛋白水通道蛋白-3 (AQP3)，并比较了与野生型细胞的克隆生存反应。结果显示，在 H2O2 治疗剂量下，siAQP3 MIA PaCa-2 细胞的克隆形成反应中的存活分数显着较高（P < 0.05）。这些结果表明过氧孔蛋白表达对于调节癌细胞对抗坏血酸治疗的敏感性具有重要意义。
Cancer cell toxicity to therapeutic H2O2 varies widely depending on cell type. Interestingly, it has been observed that different cancer cell types have varying peroxiporin expression. We hypothesize that variation in peroxiporin expression can alter cell susceptibility to therapeutic H2O2 concentrations. Here, we silence peroxiporin aquaporin-3 (AQP3) on the pancreatic cancer cell line MIA PaCa-2 and compare clonogenic survival response to the wild-type. The results showed a significantly higher surviving fraction in the clonogenic response for siAQP3 MIA PaCa-2 cells at therapeutic H2O2 doses (P < 0.05). These results suggest that peroxiporin expression is significant in modulating the susceptibility of cancer cells to ascorbate therapy.