Enrichment and characterization of hydrocarbon-degrading bacteria from petroleum refinery waste as potent bioaugmentation agent for in situ bioremediation
Enrichment and characterization of hydrocarbon-degrading bacteria from petroleum refinery waste as potent bioaugmentation agent for in situ bioremediation
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DOI：
10.1016/j.biortech.2017.05.010
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发表时间：
2017-10-01
影响因子：
11.4
通讯作者：
Sar, Pinaki
中科院分区：
文献类型：
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作者：
Sarkar, Poulomi;Roy, Ajoy;Sar, Pinaki
通过分离可培养菌株并对其特性进行研究，探讨了炼油厂废弃物中细菌的内在生物降解潜力。假单胞菌属和芽孢杆菌属。通过富集分离的菌株显示出优良的上级代谢特性：利用脂肪族（C6-C22）和多环芳烃化合物，厌氧生长，具有多个末端电子受体和较高的生物表面活性剂产量。采用GC-MS沿着检测烷烃羟化酶（alkB）基因，对十二烷的生物降解进行了研究。微生物与肠杆菌，潘多拉和伯克霍尔德菌菌株的生物增强表现出有效的生物降解（98%的TPH去除率），以及符合一级动力学模型与低速率常数和减少半衰期。这项研究证明，分解代谢效率的细菌自然存在于复杂的炼油厂废物和那些可以用于生物强化为基础的生物修复。(C)2017爱思唯尔有限公司版权所有
Intrinsic biodegradation potential of bacteria from petroleum refinery waste was investigated through isolation of cultivable strains and their characterization. Pseudomonas and Bacillus spp. populated the normal cultivable taxa while prolonged enrichment with hydrocarbons and crude oil yielded hydrocarbonoclastic bacteria of genera Burkholderia, Enterobacter, Kocuria, Pandoraea, etc. Strains isolated through enrichment showed assemblages of superior metabolic properties: utilization of aliphatic (C6-C22) and polyaromatic compounds, anaerobic growth with multiple terminal electron acceptors and higher biosurfactant production. Biodegradation of dodecane was studied thoroughly by GC-MS along with detection of gene encoding alkane hydroxylase (alkB). Microcosms bioaugmented with Enterobacter, Pandoraea and Burkholderia strains showed efficient biodegradation (98% TPH removal) well fitted in first order kinetic model with low rate constants and decreased half-life. This study proves that catabolically efficient bacteria resides naturally in complex petroleum refinery wastes and those can be useful for bioaugmentation based bioremediation. (C) 2017 Elsevier Ltd. All rights reserved.