The selenoproteome is dispensable in bloodstream forms of Trypanosoma brucei
The selenoproteome is dispensable in bloodstream forms of Trypanosoma brucei
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DOI：
10.1016/j.molbiopara.2009.08.007
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发表时间：
2009-12-01
影响因子：
1.5
通讯作者：
Schneider, Andre
中科院分区：
文献类型：
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作者：
Aeby, Eric;Seidel, Viktoria;Schneider, Andre
本研究表明，缺乏Sep-tRNA:Sec-tRNA合成酶（SepSecS）是合成三种锥虫硒蛋白所需的关键酶，并不影响布鲁氏锥虫血流形式的生长。布鲁氏锥虫的两个生命周期阶段都对黄嘌呤蛋白高度敏感，这是一种已知的靶向硒蛋白的化合物。然而，在SepSecS双敲除细胞系中观察到相同的敏感性，这表明金嘌呤蛋白的杀锥虫作用与硒蛋白无关。最后，我们发现硒蛋白的缺失不会增加对h2o2诱导的氧化应激的敏感性，因此在细胞培养中，原环和血流T细胞的正常生长不依赖于硒蛋白。(C) 2009 Elsevier B.V.版权所有
Here we show that absence of Sep-tRNA:Sec-tRNA synthase (SepSecS) a key enzyme required for the synthesis of the three trypanosomal selenoproteins does not affect growth of bloodstream forms of Trypanosoma brucei Both life cycle stages of T brucei are highly sensitive to auranofin, a compound known to target selenoproteins. However, the same sensitivity is observed in the SepSecS double knockout cell lines indicating that the trypanocidal action of auranofin is not connected to selenoproteins. Finally, we show that absence of selenoproteins does not increase sensitivity to H2O2-induced oxidative stress Thus in cell Culture normal growth of procyclic and bloodstream T brucei does not depend on selenoproteins. (C) 2009 Elsevier B.V. All rights reserved.