Fructose Promotes Crucian Carp Survival Against Aeromonas hydrophila Infection.
Fructose Promotes Crucian Carp Survival Against Aeromonas hydrophila Infection.
复制标题
果糖促进鲫鱼抵抗嗜水气单胞菌感染的存活
DOI：
10.3389/fimmu.2022.865560
复制
发表时间：
2022
影响因子：
7.3
通讯作者：
Peng B
中科院分区：
文献类型：
--
作者：
Cao Y;Kou T;Peng L;Munang'andu HM;Peng B
水生食品正成为一种重要的食物来源，为人类提供微量营养素。野生水生动物数量的减少，使得水产养殖对于发挥这一作用愈发重要。然而，传染病，尤其是细菌感染，对水产养殖构成严重威胁，造成巨大的经济损失。与此同时，目前仍缺乏不依赖抗生素来应对细菌感染的策略。在本研究中，我们监测了鲫鱼感染嗜水气单胞菌后代谢组的变化。我们发现，死于感染的鱼与存活下来的鱼，其新陈代谢存在差异。通过多变量分析，我们确定果糖是一种关键的生物标志物，在濒死组和存活组中其丰度显著不同，存活组的果糖含量高于濒死组。外源补充果糖可使鱼的存活率提高27.2%。定量基因表达分析表明，果糖能增强溶菌酶和补体3的表达，这一点在血清水平上也得到了证实。此外，溶菌酶和补体3水平的提高增强了血清细胞的裂解活性，有助于减少体内的细菌载量。因此，我们的研究展示了一种基于代谢的方法，即通过调节免疫反应来清除细菌感染，从而应对细菌感染。
Aquatic food is becoming an important food source that provides micronutrients to human beings. The decline of wild aquatic animals makes aquaculture become increasingly important to play this role. However, infectious diseases, especially bacterial infection, represent severe threat to aquaculture, which causes huge economic loss. Meanwhile, strategies in managing bacterial infection in an antibiotic-independent way are still lacking. In this study, we monitor the metabolomic shift of crucian carp upon Aeromonas hydrophila infection. We find that the metabolism of the fish that died of infection is distinct from the ones that survived. By multivariate analysis, we identify fructose as a crucial biomarker whose abundance is significantly different from the dying and surviving groups where the surviving group has a higher content of fructose than the dying group. Exogenous supplementation of fructose increases fish survival rate by 27.2%. Quantitative gene expression analysis demonstrated that fructose enhances the expression of lysozyme and complement 3 expression, which is also confirmed in the serum level. Furthermore, the augmented lysozyme and C3 levels enhance serum cell lytic activity which contribute to the reduced bacterial load in vivo. Thus, our study demonstrates a metabolism-based approach to manage bacterial infection through modulating immune response to clear bacterial infection.