Identification of an NAP-like transcription factor BeNAC1 regulating leaf senescence in bamboo (Bambusa emeiensis 'Viridiflavus')
Identification of an NAP-like transcription factor BeNAC1 regulating leaf senescence in bamboo (Bambusa emeiensis 'Viridiflavus')
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DOI：
10.1111/j.1399-3054.2011.01472.x
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发表时间：
2011-08-01
影响因子：
6.4
通讯作者：
Ding, Yulong
中科院分区：
文献类型：
--
作者：
Chen, Yunxia;Qiu, Kai;Ding, Yulong
NAC(NAM，ATAF1，2和CUC2)蛋白是植物特有的转录因子，在植物发育、非生物胁迫反应、防御和叶片衰老中发挥重要作用。本研究从毛竹‘ViridiHuang us’中分离并鉴定了一个NAC基因，初步命名为BeNAC1。序列分析表明，BeNAC1属于NAP(NAC-like，由APETALA 3/Pistillata激活)亚群，在N-末端含有保守的NAC结构域。在酵母中的转录激活分析表明，BeNAC1及其C末端具有反式激活活性。BeNAC1定位于细胞核，其转录水平与叶片衰老程度呈正相关。在AtNAP上游1.5kb片段的驱动下，BeNAC1可以挽救NAP的延缓衰老表型。BeNAC1的结构性过表达导致了拟南芥不同的早熟衰老表型。这些结果表明，BeNAC1可能在竹子叶片衰老过程中起着重要的调节作用。
NAC (NAM, ATAF1,2 and CUC2) proteins, which are plant-specific transcription factors, have crucial roles in plant development, abiotic stress responses, defense and leaf senescence. In this study, an NAC gene was isolated and characterized from the bamboo Bambusa emeiensis 'Viridiflavus' and tentatively named BeNAC1. Sequence analysis revealed that BeNAC1 belongs to the NAP (NAC-like, activated by APETALA 3/PISTILLATA) subgroup and has a conserved NAC domain in the N-terminus. Transcriptional activation analysis in yeast indicated that BeNAC1 as well as its C-terminus have trans-activation activity. BeNAC1 localized in the nucleus and its transcript level correlated positively with the severity of leaf senescence. Driven by a 1.5-kb upstream fragment of AtNAP, BeNAC1 could rescue the delayed-senescence phenotype of nap. Constitutive overexpression of BeNAC1 resulted in various precocious senescence phenotypes in Arabidopsis. These results collectively indicate that BeNAC1 might play an important regulatory role in leaf senescence in bamboo.