Localization of luteinizing hormone-releasing hormone in the preoptic area and hypothalamus of the rat using radioimmunoassay.
Localization of luteinizing hormone-releasing hormone in the preoptic area and hypothalamus of the rat using radioimmunoassay.
复制标题
使用放射免疫测定法定位大鼠视前区和下丘脑的黄体生成素释放激素。
作者：
J. E. Wheaton;L. Krulich;S. Mccann
为了确定促黄体生成素释放激素（LHRH）的定位，将5只成年雄性大鼠的脑在-10 ℃的低温恒温器中在额面、水平面或矢状面连续切片。用放射免疫分析法（RIA）和生物测定法对每个切片的乙酸-乙醇提取物进行LHRH测定。大约0.2 ng LHRH集中在内侧基底视前区（MB-PO）组织覆盖的吻侧部分的视交叉。这种LHRH似乎与终板的血管器和/或邻近的神经组织有关。下丘脑视前区（POA）和延髓正中隆起（ARC-ME）区域之间的组织中检测到均匀的低水平LHRH。在ARC-ME区，2.7 ng LHRH主要集中在正中隆起。在ARC ME区的LHRH的外侧分布延伸超出正中隆起到对应于腹内侧核的外侧的组织。LHRH的生物测定和放射免疫测定具有高度相关性。在生物测定的切片中，仅含有LHRH的切片释放FSH。这些结果证实了LHRH存在于POA和大鼠大脑的吻侧下丘脑。LHRH在POA中调节LH释放的可能意义进行了讨论。
To determine the localization of luteinizing hormone-releasing hormone (LHRH), five brains from adult male rats were serially sectioned in a cryostat at - 10 C in either the frontal, horizontal or sagittal planes. Acetic acid-ethanol extracts of each section were assayed for LHRH using radioimmunoassay (RIA) and in some cases using bioassay as well. Approximately 0.2 ng LHRH was concentrated in medial basal preoptic (MB-PO) tissue overlying the rostral portion of the optic chiasm. This LHRH appears to be associated with the organum vasculosum of the lamina terminalis and/or adjacent neural tissue. Uniform, low levels of LHRH were detected in hypothalamic tissue between the preoptic area (POA) and arcuate-median eminence (ARC-ME) region. In the ARC-ME region 2.7 ng of LHRH were concentrated primarily in the median eminence. The lateral distribution of LHRH in the ARC-ME region extended beyond the median eminence into tissue corresponding to the lateral aspect of the ventromedial nucleus. Concomitant bioassay and RIA determinations of LHRH were highly correlated. Of the sections bioassayed, only those sections containing LHRH released FSH. These results confirm the presence of LHRH in the POA and in the rostral hypothalamus of the rat brain. The possible significance of LHRH in the POA for the regulation of LH release is discussed.