Cytokine Expression Profile in Aqueous Humor and Sera of Patients with Acute Anterior Uveitis
Cytokine Expression Profile in Aqueous Humor and Sera of Patients with Acute Anterior Uveitis
复制标题
急性前葡萄膜炎患者房水和血清中细胞因子的表达谱
DOI：
10.2174/1566524015666150731100012
复制
发表时间：
2015-01-01
影响因子：
2.5
通讯作者：
Chen, L.
中科院分区：
文献类型：
--
作者：
Chen, W.;Zhao, B.;Chen, L.
目的：评价HLA-B27相关性急性前葡萄膜炎（AAU）和特发性AAU患者房水和血清中细胞因子的表达谱。方法：2012年8月至2013年3月，选取AAU患者20例，对照组17例。将葡萄膜炎患者分为两组：特发性AAU患者9例，HLA-B27相关AAU患者11例。进行了完整的眼科检查，并清楚地记录了各组的临床特征。收集房水和血清，采用多重免疫分析法同时测定15种免疫介质（IL-1 β、IL-4、IL-6、IL-10、IL-17A、IL-17F、IL-21、IL-22、IL-23、IL-25、IL-31、IL-33、tnf - α、ifn - γ、sCD40L）在房水和血清中的浓度。结果：与对照组相比，葡萄膜炎患者房水中多种细胞因子水平显著升高，包括IL-1 β、IL-6、IL-10、IL-17a、IL-17f、IL-21、IL-25、IL-31、ifn - γ、tnf - α和sCD40L。在特发性AAU患者中，房水中IL-17a水平与疾病活动性显著相关，而在HLA-B27相关性AAU患者中，房水中ifn - γ水平与疾病活动性显著相关。除IL-6在特发性和HLA-B27相关AAU患者中均升高外，葡萄膜炎患者与对照组血清细胞因子表达无显著差异。结论：房水细胞因子表达模式与血清细胞因子表达模式不同，可反映AAU患者活动性炎症期间的眼内免疫反应。Th1和Th17都参与HLA-B27相关和特发性AAU的免疫发病机制，但在这两种临床实体中发现了不同的细胞因子模式。th17主导的免疫反应可能在特发性AAU的免疫发病机制中发挥重要作用，而Th1主导的免疫反应可能是HLA-B27相关AAU炎症的原因。
Purpose: To evaluate cytokine expression profile in aqueous humor and sera in patients with HLA-B27 associated acute anterior uveitis (AAU) and idiopathic AAU.Methods: Twenty patients with AAU and 17 controls were recruited from August 2012 to March 2013. Study subjects with uveitis were divided into two groups: 9 patients with idiopathic AAU and 11 patients with HLA-B27 associated AAU. Complete ophthalmological examinations were performed and clinical features of each group were clearly documented. Aqueous humor and sera were collected and the concentration of 15 immune mediators (IL-1 beta, IL-4, IL-6, IL-10, IL-17A, IL-17F, IL-21, IL-22, IL-23, IL-25, IL-31, IL-33, TNF-alpha, IFN-gamma, sCD40L) were measured in both aqueous humor and sera simultaneously by multiplex immunoassay.Results: There were significantly higher levels of multiple cytokines in aqueous humor in patients with uveitis compared to controls, including IL-1 beta, IL-6, IL-10, IL-17a, IL-17f, IL-21, IL-25, IL-31, IFN-gamma, TNF-alpha, and sCD40L. The levels of IL-17a in aqueous humor correlated significantly with disease activity in patients with idiopathic AAU, while the level of IFN-gamma in aqueous humor correlated significantly with disease activity in patients with HLA-B27 associated AAU. There was no significant difference in serum cytokine expression between uveitis patients and controls except IL-6, elevated in patients with both idiopathic and HLA-B27 associated AAU.Conclusion: Cytokine expression pattern in the aqueous humor, in contrast to that in serum, may reflect intraocular immune reactions during active inflammation in patients with AAU. Both Th1 and Th17 are involved in immunopathogenesis of HLA-B27 associated and idiopathic AAU, but a different cytokine pattern was identified in these two clinical entities. A predominant Th17-driven immune response may play an important role in the immunopathogenesis of idiopathic AAU, while Th1 dominant immune response may be responsible for the inflammation in HLA-B27 associated AAU.