Chronic exposure of Sk-1 hairless mice to narrow-band ultraviolet A (320-355 nm)
Chronic exposure of Sk-1 hairless mice to narrow-band ultraviolet A (320-355 nm)
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Sk-1无毛小鼠长期暴露于窄带紫外线A（320-355 nm）
DOI：
10.1111/j.1600-0781.1996.tb00236.x
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Willis,I
中科院分区：
文献类型：
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作者：
Menter,JM;Sayre,RM;Etemadi,AA;Agin,PP;Willis,I
最近的几项研究共同表明，紫外线A（UVA）在慢性光化性皮肤损伤中的作用可能比最初认为的要大。在目前的工作中，氙弧太阳模拟器的输出通过Bausch & Lomb单色器与2 mm Schott WG-320滤光片结合产生中心在338 nm的窄带UVA，半带宽24 nm，Io=3.4±0.3 mW/cm 2。我们对10只Sk-1白化病无毛小鼠进行长期照射，每周5次，持续18周，从1.25 J/cm 2开始，照射33天，随后依次为1.50 J/cm 2（34天）、1.8 J/cm 2（10天）、2.0 J/cm 2（22天），在99个照射日内提供154.3 J/cm 2的总UVA剂量。在第6天临床上观察到红斑，其在整个照射期间持续存在。在辐照期间，在辐照第37-56天，观察到与轻度表皮增生一致的一些鳞屑。尽管持续的慢性照射，这种反应后来消退。最后一次照射后立即进行苏木精和伊红检查，发现轻度炎症反应，伴有一些真皮重建。在实验结束时，没有观察到表皮增生或（前）恶性病变的显著迹象，尽管注意到一些角质层增厚。明显的真皮胶原蛋白损伤和中度弹性组织变性也很明显。我们认为，在以前的研究中报告的结果中观察到的差异在很大程度上是由于光源和照射方案的差异。
Several recent investigations collectively suggest that the role of ultraviolet A (UVA) in chronic actinic skin damage may be greater than originally thought. In the present work, the output of a xenon‐arc solar‐simulator passed through a Bausch & Lomb monochromator in conjunction with a 2‐mm Schott WG‐320 filter produced narrow‐band UVA centered at 338 nm, half‐band width 24 nm, Io=3.4±0.3 mW/cm2. We chronically irradiated 10 Sk‐1 albino hairless mice 5 times per week for 18 weeks, starting with 1.25 J/cm2, for 33 irradiation days, sequentially followed by 1.50 J/cm2(34 days), 1.8 J/cm2(10 days), 2.0 J/cm2(22 days) to afford a total UVA dose of 154.3 J/cm2over 99 irradiation days. Erythema was noted clinically by day 6, which persisted throughout the irradiation. During the irradiation period, some scaling, consistent with mild epidermal hyperplasia was noted during irradiation days 37–56. This response later regressed despite continued chronic irradiation. Hematoxylin and eosin examination immediately after the final irradiation revealed a mild inflammatory response, with some dermal restructuring. At the end of the experiment, no significant signs of epidermal hyperplasia or (pre)malignant lesions were seen, although some stratum corneum thickening was noted. Marked dermal collagen damage and moderate elastosis was also evident. We believe that the observed differences in results reported in previous studies are in large part due to differences in light sources and irradiation protocols.